background: ct scan and nuclear medicine exams deliver a great part of medical exposures. this study examined professional radiation hazards in ct scan and nuclear medicine workers.   methods : in a cross sectional study 30 occupationally exposed workers and 7 controls (all from personnel of a laboratory) were selected. physical dosimetry was performed for exposed workers. blood samples were ob...

The technique of pulse-field gel electrophoresis (PFGE) makes use of restriction enzymes that cleave DNA at specific restriction sites, generating DNA fragments of different sizes. These fragments can be separated according to size by agarose-gel electrophoresis and visualized using DNA stains (e.g. ethidium bromide), giving a characteristic banding pattern. Given the high specificity of restri...

BACKGROUND The recent article by Guller, Klein, Hagen was reviewed and discussed by the authors of this response to critically analyze the validity of the conclusions, at a time when patients and providers depend on peer reviewed data to guide their health care choices. The authors of this response all have high volume bariatric surgery practices encompassing experience with both gastric bypass...

Nearly all methods of chromosome banding rely on harvesting chromosomes in mitosis. This is usually achieved by treating cells with tubulin inhibitors, such as colchicine or demecolcine (colcemid), that depolymerize the mitotic spindle and so arrest the cell at this stage. Excessively long incubations with Colcemid result in overcondensed chromosomes that band poorly and moreover some cell type...

OBJECTIVE Hypoplastic left heart syndrome remains a challenge for worldwide surgeons. Initial palliation employing bilateral pulmonary artery banding along with ductal stent implantation and atrial septostomy has been proposed as an alternative approach. However, the surgically placed bands are fixed and may become inadequate after sternum closure or with somatic growth of the patient. We descr...

AIMS Inhibition of phosphodiesterase 5 (PDE5) decreases pulmonary pressure and improves symptoms in patients with pulmonary arterial hypertension. It is unclear however, whether inhibition of PDE5 can prevent myocardial remodelling during right-ventricular pressure overload. METHODS AND RESULTS Right-ventricular pressure overload was produced in male rats in a pulmonary hypertension model (mo...

Mixed gonadal dysgenesis (MGD) is an abnormal sexual differentiation syndrome usually presenting with ambiguous genitalia. Karyotype analysis is one of the essential components in the diagnosis of MGD and is conventionally done with peripheral lymphocytes by the G-banding technique. It is speculated that this conventional karyotype analysis has limitations since there are often difference in go...

Objectives: To elucidate the role of KLF15 on cardiac fibroblast in the dynamic angiogenesis in pressureoverload cardiac remodeling. Methods: Aortic banding was performed in rats to induce pressure-overload and cardiac hypertrophy. Part of the animals has their aorta constriction removed 3 or 6 weeks after the banding surgery. Cardiac function by echocardiography, cardiac tissue morphology and ...

AIM It was the aim of this study to investigate the long- term effects of reduced aortic compliance on cardiovascular hemodynamics and cardiac remodeling. METHOD Sixteen swine, divided into 2 groups, a control and a banding group, were instrumented for pressure and flow measurement in the ascending aorta. Teflon prosthesis was wrapped around the aortic arch in order to limit wall compliance i...

Dialysis-associated steal syndrome (DASS) is defined as a clinical condition caused by arterial insufficiency distal to the dialysis access owing to diversion of blood into the fistula or graft. The incidence of symptomatic DASS requiring treatment is 1-8%. The etiology is iatrogenic and symptoms are quite debilitating. Banding of the access inflow has largely been abandoned because of the inhe...