Prolonged drought results in serious ecological consequences forest ecosystems, particularly for soil microbial communities. However, much is unknown about communities their response to long-term consecutive droughts warm-temperate forests. Here, we conducted a 7-year manipulated throughfall reduction experiment (TFR) examine the responses of bacterial and fungal terms richness networks. Our sh...

In the mid-50s of last century, reports began to appear about ineffectiveness sulfonamides and antibiotics in bacterial infections. And soon widespread spread numerous strains antibiotic-resistant microorganisms was noted. The fight against drug-resistant bacteria is carried out several directions: obtaining new chemotherapeutic drugs, chemical modification known antibiotics, or use inhibitors ...

A circular map of the 208-kilobase IncHII plasmid pHH1508a was constructed by using the restriction endonucleases XbaI, XhoI, and NotI. Tn5 insertion mutagenesis showed that transfer genes were distributed widely over the plasmid map. Analysis of the deletion mutant pDT1178 indicated that genes required for conjugative pilus production are located in a 96.0-kilobase region of the plasmid.

Total DNA from five Campylobacter species was digested with a mixture of XhoI and BglII restriction endonucleases and analyzed by Southern hybridization by using a probe complementary to the DNA coding for the 16S rRNA. Each of the Campylobacter species, including C. jejuni, C. coli, C. laridis, C. fetus, and C. upsaliensis, displayed a characteristic pattern. Although some bands may be common ...

The chromosomal DNA of four strains of Gardnerella vaginalis were digested with rare cutting restriction enzymes and analyzed by pulsed-field gel electrophoresis (PFGE). The four strains studied were two clinical isolates (GVP 004 & GVP 007) and two American Type Culture Collection strains (ATCC 14018 & ATCC 14019). The restriction enzyme SfiI generated two DNA fragments of about 0.6 Mb and 1.1...

CLONEPICKER is a software pipeline that integrates sequence data with BAC clone fingerprints to dynamically select a minimal overlapping clone set covering the whole genome. In the Rat Genome Sequencing Project (RGSP), a hybrid strategy of "clone by clone" and "whole genome shotgun" approaches was used to maximize the merits of both approaches. Like the "clone by clone" method, one key challeng...

the prospects of using the biostimulation and bioaugmentation treatment for the removal of the polychlorinated biphenyl (pcbs) from contaminated sediment collected from the sewage canal in the surroundings of a former pcb manufacturing plant in slovakia is described. the eleven bacterial strains isolated in our previous work from sewage strážsky canal sediments were able to aerobically degrade ...

Bioactive marine natural products can be defined as biologically active products, both secondary metabolites as well as enzymes, lipids and heteropolysaccharides derived from marine sources. Marine natural products have been found to be an important source of drugs and drug leads. These natural products are secondary metabolites and enhance survival fitness and may serve as chemical weapons use...