Strain EB01(T) sp. nov. is the type strain of Bacillus massilioalgeriensis, a new species within the genus Bacillus. This strain, whose genome is described here, was isolated from sediment sample of the hypersaline lake Ezzemoul sabkha in northeastern Algeria. B. massilioalgeriensis is a facultative anaerobic Gram-positive bacillus. Here we describe the features of this organism, together with ...

Flourene and phenanthrene are organic compounds with high hydrophobicity and toxicity. Being recalcitrant in nature they are accumulating in the environment at an alarming concentration, posing serious threat to living beings. Thus in the present study, microorganisms were screened for their ability to degrade these contaminants at high concentrations in least period of time. Two out of fifteen...

Bacillus sp. strain W2 is a plant growth-promoting rhizobacterium isolated from saffron fields of Kashmir, India. Here, we report the draft genome sequence (3.9 Mb) of Bacillus amyloliquefaciens strain W2 having 65 contigs (3, 997, 511 bp), 4,163 coding sequences, and an average 46.45% GC content. Despite the 99% identity of the 16S rRNA gene with that of Bacillus amyloliquefaciens subsp. plant...

In this research, antagonistic activity of two Bacillus species isolated from digestive tract of marine fish and culture pond of sea cucumber was studied. The inhibitory activity of Bacillus spp. isolates against some common pathogenic bacteria of fish was assessed using the agar diffusion method. The strain of B. subtilis G024 exhibited antimicrobial activity against Vibrio anguillarum, V. har...

Crude extracts were prepared using the solvent ethanol from the seaweeds viz., Gracilaria edulis, Calorpha peltada and Hydroclothres sp. and screening for their antibacterial activity against six bacterial pathogens were also carried out. The test bacterial strains were Escherichia coli, Enterobacter aerogenes, Staphylococcus aureus, Pseudomonas aeruginosa, Streptococcus faecalis and Bacillus c...

The relative isolation and unique physical properties of the Savusavu Hot Springs in Fiji may yield unique thermophiles. This study was conducted to determine the presence of aerobic thermophilic bacteria in these hot springs. A total of 104 thermophilic bacterial isolates were characterized and using Thermus and Bacillus strains as controls, 58% of the isolates were identified as Anoxybacillus...

A 1.5 Å resolution X-ray structure of the catalytic module of Caldicellulosiruptor bescii family 3 pectate lyase is reported (PDB entry 3t9g). The resulting structure was refined to an R factor of 0.143 and an R(free) of 0.178. Structural analysis shows that this new structure is very similar to the previously solved structure of a family 3 pectate lyase from Bacillus sp. strain KSM-P15 (PDB en...

The bioflocculant-producing potentials of a marine bacteria isolated from the bottom sediment of Algoa Bay was investigated using standard methods. The 16S rDNA sequence analysis revealed 98% similarity to that of Bacillus sp. HXG-C1 and the nucleotide sequence was deposited in GenBank as Bacillus sp. Gilbert with accession number HQ537128. Bioflocculant was optimally produced when sucrose (72%...

ManH, a novel substrate-binding protein of an ABC transporter, was identified from the mannan utilization gene cluster of Bacillus sp. N16-5. We cloned, overexpressed, and purified ManH and measured its binding affinity to different substrates by isothermal titration calorimetry. ManH binds to mannotriose, mannotetraose, mannopentose, and galactosyl-mannotriose with dissociation constants in th...