Several nonpeptide small molecules were designed as potential inhibitors of HIV protease and their structures were constructed by computer-aided molecular modeling and docked iwo the active site of HIV protease. Models of the complexes of inhibitors and the HIV protease were refined using nonbonded and H-bonding terms. The refined energy of selected complexes showed that the designed inhib...

The stimulated stimulated radiation and absorption theory of light based on low order linear interaction is considered symmetrical in time reversal processes at present. It is provided in the paper that after high order nonlinear interaction between radiation fields and charged particles is considered, time reversal symmetry in the stimulated radiation and absorption processes would be violated...

hydatidosis is a zoonosis disease caused by the larva of echinococcus granulosus parasite in man and animals. the proteases enzymes are necessary for nutrition, migration and evasion of immunity of parasite into host. the aim of this study was to determine the protease activity of hydatid cyst protoscolices (psc), healthy and cystic liver tissues in order to compare of this biomarker for host a...

proteases are important enzymes that their role in various industries is undeniable. however, keeping enzymes stable during its activity in harsh conditions is so important. in this study, protease enzyme was immobilized on the porous silica particles and its stability in different temperatures and phs was evaluated. first silica particles were aminated by 3-aminopropyltriethoxysilane then the ...

We report on the use of recombinant human intrinsic factor (rhIF) in a new vitamin B12 absorption test. Holotranscobalamin (holoTC) was measured before and 24 hours after intake of three 9-mg doses of vitamin B12 (B12) and again 24 hours after intake of the same dose of B12 together with rhIF (rhIF-B12). Nine patients with evident vitamin B12 deficiency had a significantly higher increase in ho...

Human immunodeficiency virus type 1 (HIV-1) protease inhibitors comprise an important class of drugs used in HIV treatments. However, mutations of protease genes accelerated by low fidelity of reverse transcriptase yield drug resistant mutants of reduced affinities for the inhibitors. This problem is considered to be a serious barrier against HIV treatment for the foreseeable future. In this st...

p AST research pertinent to vitamin B12, streptomycin, or aureomycin in pig nutrition has been critically reviewed in a recent paper by Briggs and Beeson (1951). In this same paper, the authors definitely showed that Lederle's B12-aureomycin feeding supplement produced a greater growth response in pigs than Merck's B12 concentrate. Since the two supplements were fed to supply about the same vit...

optimization of the fermentation medium for maximum alkaline protease production was carried out. fifteen positive isolates were examined for their extent of alkaline protease production. the most potent producer was identified as bacillus sp. the solid substrate screening showed that the combination of wheat straw and bean husk was the best one. the initial screening by using plackett–burman’s...

in the detergent industry. In this study, the extracellular alkaline serine protease gene, aprE, from Bacillusclausii was amplified by PCR and further cloned and expressed in B. subtilis WB600 using the pWB980 expression vector. Protease activity of the recombinant B. subtilis WB600 harboring the plasmid pWB980/aprEreached up to 1020 U/ml, approximately 3-folds higher than the nativ...