Background: Helicobacter pylori (HP) infection is one of the most important causes of gastrointestinal diseases such as peptic ulcer. This bacterium has recently been reported to be associated with low serum vitamin B12 level. In this study we investigated the relationship between Helicobacter pylori seropositivity and the serum B12 level in women aging 15-49 years in Khorram Abad . Material...

IN EARLIER WORK'. 2 the metabolism of vitamin B12 was studied in pa-tietits with pernicious and other nutritional anemias. The microbiological assay with E. coli mutant' and Euglena gracilis2 was used for the quantitation of vitamin B12 in urine and blood, following injection or oral administration of vita-mm B12 alone or with various gastric materials of humaii or animal origin, coi'-taming in...

A reference radioassay for vitamin B12 in serum, in which human serum is used as binder and DEAEcellulose is used to separate free and bound B12, was compared (194 sera) with a packaged kit (“Phadebas B12 Test”), in which intrinsic factor is used as binder and poiysaccharide for separation. The correlation coefficient was 0.949. With the kit, added B12 was identifiable, proportional dilution ch...

A sensitive and selective method is needed to analyse in situ produced vitamin B12 in plant-based materials, potential new dietary sources of vitamin B12. A UHPLC/UV method was developed and validated for the determination of human active vitamin B12 in cell extracts of Propionibacterium freudenreichii subsp. shermanii and after immunoaffinity purification in extracts of cereal matrices ferment...

A radioisotope dilution assay for vitamin B12, using hemoglobin-coated charcoal, was modified in several respects, the most prominent of which was the use of cyanide extraction of sera. With these modifications, the B12 levels were significantly higher in sera from normal subjects and patients with folate deficiency, whereas sera from patients with pernicious anemia and other B12-deficient stat...

The natural vitamin B12-derivatives are intriguing complexes of cobalt that entrap the metal within strikingly skewed and ring-contracted corrin ligand. Here, we describe synthesis Rh(III)-corrin acetylrhodibalamin (AcRhbl) from biotechnologically produced metal-free hydrogenobyric acid analyze effect replacement cobalt-center organometallic B12-derivative acetylcobalamin (AcCbl) with its group...

BACKGROUND The fraction of total plasma vitamin B(12) bound to transcobalamin (holoTC/B12 ratio) may reflect tissue levels of the vitamin, but its clinical relevance is unclear. METHODS We assessed associations between cognitive function and total B12, holoTC, and holoTC/B12 ratio in a cohort of elderly Latinos (n = 1089, age 60-101 years). We assessed cognitive function using the Modified Mi...

This paper evaluates the possible reasons for consistent vitamin B12 deficiency in chronic toxigenic mold exposures and the synergistic relationships with the possible mycotoxic effects on one-carbon metabolism that lead to the manifestations of clinical neuropathological symptomology. Vitamins are first defined in general and the nutritional sources of vitamin B12 are evaluated in particular. ...