Polyphosphate (polyP) is an anionic polymer of orthophosphate groups linked by high energy bonds that typically accumulates in acidic, calcium-rich organelles known as acidocalcisomes. PolyP synthesis in eukaryotes was unclear until it was demonstrated that the protein named vacuolar transporter chaperone 4 (Vtc4p) is a long chain polyP kinase that localizes to the yeast vacuole. Here, we repor...

Polyphosphate (polyP) is a physiologically occurring polyanion that is synthesized especially in bone-forming osteoblast cells and blood platelets. We used amorphous polyP nanoparticles, complexed with Ca(2+), that have a globular size of ∼100 nm. Because polyP comprises inorganic orthophosphate units that are linked together through high-energy phosphoanhydride bonds, we questioned whether the...

PURPOSE To analyze the correlation of clinical symptom and endoscopic-pathological characteristics of colorectal polyps. METHODS A retrospective study was performed on 1,234 continuous colorectal polyp patients. Their clinical, colonoscopic and pathological data were collected and analyzed. RESULTS In 1,234 patients, 46.0% cases were asymptomatic, and 54.0% cases were symptomatic, and the f...

PolyP (inorganic polyphosphate) is a linear polymer of many tens or hundreds of orthophosphate residues found in a wide range of organisms, including bacteria, fungi, insects, plants and vertebrates. Despite its wide distribution in mammalian tissues and plasma, the biological functions of polyP on tumour metastasis and angiogenesis have not been previously examined. In the present study, we ha...

Inflammation is a hallmark of colorectal cancer (CRC). Neutrophils are well-known mediators in tumor biology but their role in solid tumors, including CRC, was redefined by neutrophil extracellular traps (NETs). Given that it was recently demonstrated that platelet-derived polyP primes neutrophils to release NETs, we examined surgical specimens from CRC to investigate the presence of polyP, as ...

Escherichia coli transiently accumulates large amounts of inorganic polyphosphate (polyP), up to 20 mM in phosphate residues (Pi), in media deficient in both Pi and amino acids. This transient accumulation is preceded by the appearance of nucleotides ppGpp and pppGpp, generated in response to nutritional stresses. Mutants which lack PhoB, the response regulator of the phosphate regulon, do not ...

Polyphosphate (polyp) kinase was prepared from EDTA-lysozyme spheroplasts of a Salmonella minnesota rough mutant. The enzyme, purified about 8o-fold, was free of ATPase and polyp-degrading activity and contained neither nucleic acids nor polyp which might act as primer. Using this enzyme, 32P-labelled polyp was prepared from [?P]ATP. The polyp was identified by the action of degrading enzymes, ...

BACKGROUND Although cochlear implantation has been almost a standard otological procedure worldwide, it may still create a dilemma for the surgeon in some unusual instances such as Seckel syndrome, aural atresia and posterior fossa arachnoid cyst. CASE REPORT Three extraordinary cases of cochlear implantation were reported. The first case was a case of Seckel syndrome with a cardiac pacemaker...

results methylation in at least one gene was reported about 93.8%. the methylation of sfrp2 was reported in 39 out of the 48 subjects (81.2%) whereas methylation of mgmt was observed in 32 subjects (66.6%). conclusions the current study results showed that methylation of sfrp2 and mgmt play an important role in polyp formation. materials and methods forty eight tissue samples of polyp and adeno...

This chapter describes various techniques that can be employed to assist in the removal of colon polyps that are considered to be large or ‘difficult.’ Size alone is only one of the features that may cause some hesitation in making the decision to attempt polyp removal. Other factors that are related to the perceived level of difficulty are polyps that are flat and only slightly elevated above ...