Duman, Joseph G., Kamala Tyagarajan, Michelle S. Kolsi, Hsiao-Ping H. Moore, and John G. Forte. Expression of rab11a N124I in gastric parietal cells inhibits stimulatory recruitment of the H1-K1-ATPase. Am. J. Physiol. 277 (Cell Physiol. 46): C361–C372, 1999.—Stimulation of the gastric parietal cell results in a massive redistribution of H1-K1-ATPase from cytoplasmic tubulovesicles to the apica...

Abstract Background and Aims The ATP6V0A4 gene is localized to chromosome 7q33∼34 encodes for vacuolar H+-ATPase (V-ATPase) α4 subunit. Decreased V-ATPase function due mutations in the genes could cause H+ excretion defect of α-intercalated cells renal collecting duct, resulting hereditary distal tubular acidosis (dRTA). However, crucially, no gain-of-function have been reported previously. Thi...

Abstract Hydrogen sulfide (H 2 S) can alleviate Cd-induced cell death, but the molecular mechanisms are not clear. To shed light on these mechanisms, death induced by 200 μM cadmium chloride in cucumber seedlings root tips was used as a model system. Here, we report that negative effect of Cd stress mitochondrial physiological functions include changes cytochrome c/a, membrane permeability tran...

Duman, Joseph G., Kamala Tyagarajan, Michelle S. Kolsi, Hsiao-Ping H. Moore, and John G. Forte. Expression of rab11a N124I in gastric parietal cells inhibits stimulatory recruitment of the H1-K1-ATPase. Am. J. Physiol. 277 (Cell Physiol. 46): C361–C372, 1999.—Stimulation of the gastric parietal cell results in a massive redistribution of H1-K1-ATPase from cytoplasmic tubulovesicles to the apica...

Alterations of tyrosine hydroxylase activity in various regions of brain from rats postnatally exposed to lead were tested. Three groups of animals were prepared; (1) Rats exposed to lead at a low dose (0.05% lead acetate, PbAc); (2) Rats exposed to lead at a high dose (0.2% PbAc); (3) Age-matched normal control rats. At 2, 4, 6, and 8 weeks of age, weight of brain and body, and concentrations ...

1. The activities of some membrane-bound enzymes such as adenylate cyclase, Na+ + K+-stimulated adenosine triphosphatase (Na+ + K+-ATPase), Ca2+-stimulated ATPase and Mg2+-stimulated ATPase were examined in heart sarcolemmal fractions from control and cardiomyopathic hamsters at different stages of heart failure. 2. The basal adenylate cyclase activity in sarcolemma from cardiomyopathic animals...

1. The initial rapid phase of ATP hydrolysis by bovine heart submitochondrial particles or by soluble F1-ATPase is insensitive to anion activation (sulphite) or inhibition (azide). 2. The second slow phase of ATP hydrolysis is hyperbolically inhibited by azide (Ki approximately 10(-5) M); the inosine triphosphatase activity of submitochondrial particles or F1-ATPase is insensitive to azide or s...

In order to evaluate the possible role of sodium- and potassium-activated adenosine triphosphatase in the active transport of sodium by the renal tubules, we examined the effect of large changes in the tubular reabsorptive load of sodium on the Na-K-ATPase activity of rat kidney homogenates. Glomerular filtration and tubular reabsorption of sodium per gram of kidney tissue increased progressive...

Effect of alcohol-kolanut interaction on sodium pump activity in wistar albino rats was studied. Thirty wistar albino rats were divided into six groups of five (5) rats per group and used for the study. The control group (1) received via oral route a placebo (4 ml of distilled water). Groups 2 to 6 were treated for a period of 21 days, with (10% v/v) of alcohol (group 2), 50mg/kg body weight of...

We studied product inhibition of the actin-activated ATPase of myosin subfragment-1 (S-1) from the three types of muscle tissue: skeletal, cardiac, and smooth. Increasing levels of [MgADP] in the 0-1-mM range caused significant inhibition of the actin-activated MgATPase activity of cardiac and gizzard but not skeletal muscle S-1. When total nucleotide concentration ([ATP] + [ADP]) was kept cons...