the carob moth is one of the most devastating pests of pomegranate and some other products. various pest control measures have been undertaken in order to control this pest but none of them has been successful so far. in the current study the effects of cereal seed proteinaceous extracts including triticale and three wheat cultivars (mv17, aflak, and zare) have been studied on α-amylase and pro...

An account of previous work on cathepsin may be found in the monograph of Pozzi (1935). In the hemoglobin method for the estimation of protelnase, denatured hemoglobin is digested and the digestion products not precipitable by trichloracefic acid are estimated colorimetrically. Hemoglobin, unlike commercial casein, edestin, and gelatin, is a reproducible substrate, Only the very first stages of...

Increasing levels of inhibitors that target cysteine and/or serine proteinases were fed to Tribolium castaneum larvae, and the properties of digestive proteinases were compared in vitro. Cysteine proteinases were the major digestive proteinase class in control larvae, and serine proteinase activity was minor. Dietary serine proteinase inhibitors had minimal effects on either the developmental t...

Bronchoalveolar lavage is used to obtain cells and proteins from the lower respiratory tract for diagnosis and research. Uncertainity exists about which site in the lung is sampled by the lavage fluid and what effect different lavage volumes have on recovery of the constituents of lavage fluid. Dilution of alveolar lining fluid by lavage fluid is variable and results are usually expressed as pr...

Antineutrophil cytoplasmic autoantibodies (ANCAs) recognizing human proteinase 3 of neutrophil granules are a diagnostic hallmark of Wegener granulomatosis, an autoimmune systemic vasculitis with predilection for the respiratory tract and kidneys. In vitro experiments have implicated several mechanisms by which ANCAs may lead to tissue injury. However, little is known about the pathogenic signi...

Background: Candida species is a normal commensal flora of human body inhabiting the skin, mucous membrane and gastro intestinal tract but may be associated with superficial deep-seated fungal infections. The switch from to potent pathogen, facilitated by various extracellular hydrolytic enzymes. aim this study was estimate phospholipase, proteinase, haemolysin esterase activity determine antif...

The effects of therapeutically relevant concentrations of the human immunodeficiency virus (HIV) proteinase inhibitors saquinavir and indinavir on the in vitro proteinase activity of Candida albicans were investigated with isolates from HIV-infected and uninfected patients with oral candidiasis. After exposure to the HIV proteinase inhibitors, proteinase activity was significantly reduced in a ...

1. Streptococcal proteinase is derived from an inactive precursor found in culture filtrates of proteinase-producing streptococci. 2. The precursor can be converted into the proteinase by low concentrations of trypsin but not by chymotrypsin. 3. In cultures grown in suitable media the conversion of precursor to proteinase is effected autocatalytically. This reaction occurs under reducing condit...

The present study intended to verify activities of cysteine proteinase of Pneumocystis carinii from rats and to purify the enzyme. In order to exclude the contamination of host-derived enzymes, concentrates of P. carinii was primarily treated with a mixture of proteinase inhibitors before lysis of P. carinii. A 68-kDa cysteine proteinase was finally purified from the crude extract of P. carinii...

The interaction ae-macroglobulin with four proteinases has been investigated by binding assays and by gel electrophoresis. At pH 7.65 the binding ratios of the proteinase-cwa-macroglobulin complexes were found to be 2:l (trypsin and papain), 1.4:1 (chymotrypsin), and 1:l (plasmin). The progressive decrease in the stoichiometry of the three seryl proteinase complexes was paralleled by a concomit...