Introduction: Ascorbic acid’s important role in collagen synthesis is well established. Since humans lack a functional gulonolactone oxidase (GLO) gene for synthesizing ascorbic acid, this nutrient must be provided in the diet and then transported into the chondrocyte for usage. Guinea pigs are one of the few non-primate mammals that also lack the GLO gene so they provided an ideal model for th...

This study presents a new approach that can stabilize effectively L-ascorbic acid in water-in-oil-in-water (w/o/w) double emulsions. Basically, the behavior of L-ascorbic acid in the aqueous phase was observed, considering its molecular deformation. Then, it was found that the stability determined in the aqueous phase by high-performance liquid chromatography (HPLC) showed that the collapse of ...

An optode was designed for an indirect determination of ascorbic acid by using neocuproine, which has been coated on transparent triacetylcellulose film as a membrane. The proposed method is based on the oxidation of ascorbic acid to dehydroascorbic acid with the Cu(II) and neocuproine reagent. The increase in the absorbance value of the optode at the maximum wavelength of 455 nm was related ...

Determinations of the concentration of ascorbic acid in whole blood (1, 2, 3, 4, 5, 6, 7, 8) and in plasma or serum (9, 10, 11, 12, 13, 14, 15, 16, 17, 18) as well are believed to indicate the degree of saturation of the organism. No strict correlation, however, has been established between the ascorbic acid content of whole blood and of plasma, although the concentration in cells is consistent...

The purposes of this study were twofold: (1) to examine the effects of bleaching on the bond strength of an adhesive to bovine tooth surfaces; and (2) to explore the effectiveness of an ascorbic acid application in preventing the deterioration of bonding ability due to bleaching. In the experimental groups, ascorbic acid was applied to the bleached bovine tooth surfaces. In the control groups, ...

Cyclophosphamide is an anticancer drug with immunosuppressive activities. Considering various reports on the possible antioxidant/protective functions of ascorbic acid, it is aimed at to explore the modulatory effect of ascorbic acid on therapeutic efficacy as well as mutagenic potentials of cyclophosphamide in the same tumor bearing mice. Swiss albino mice with ascites Dalton’s lymphoma were u...

IN an earlier communication [Birch and Dann, 1933] on the estimation and distribution of ascorbic acid and glutathione in animal tissues we drew attention to the distribution of ascorbic acid in organs other than the suprarenal cortex, as detected by the indophenol reagent. The presence in a number of tissues of reducing substances other than glutathione had become evident from the work of Maso...

High concentration of ascorbic acid (vitamin C) has been found in corneal epithelium of various species. However, the specific functions and mechanisms of ascorbic acid in the repair of corneal epithelium are not clear. In this study, it was found that ascorbic acid accelerates corneal epithelial wound healing in vivo in mouse. In addition, ascorbic acid enhanced the stemness of cultured mouse ...

BACKGROUND Extended exposure to low levels of lead causes high blood pressure in human and laboratory animals. The mechanism is not completely recognized, but it is relatively implicated with generation of free radicals, oxidant agents such as ROS, and decrease of available nitric oxide (NO). In this study, we have demonstrated the effect of ascorbic acid as an antioxidant on nitric oxide metab...

Inhibition of neuronal cyclooxygenase-2 (COX-2) and hence prostaglandin E2 (PGE2) synthesis by non-steroidal anti-inflammatory drugs has been suggested to protect neuronal cells in a variety of pathophysiological situations including Alzheimer's disease and ischemic stroke. Ascorbic acid (vitamin C) has also been shown to protect cerebral tissue in a variety of experimental conditions, which ha...