Lipid protein interactions play a key role in the stability and function of various membrane proteins. Earlier we have reported the extreme thermal stability of porin from Paracoccus denitrificans reconstituted into liposomes. Here, we used Fourier transform infrared spectroscopy for a label free analysis of the global secondary structural changes and local changes in the tyrosine microenvironm...

The major outer membrane protein of Rhodobacter capsulatus 37b4 (capsule-free) was isolated. Strong porin-activity was observed after reconstitution into artificial lipid bilayer membranes with a single channel conductance of 3.15 nS in 1 M KCl. The porin migrated as a broad, single band (Mr above 90,000) on sodium dodecyl sulfate polyacrylamide gel electrophoresis and dissociated into a single...

Building dependable distributed systems from commercial off-the-shelf components is of growing practical importance. For both cost and production reasons, there is interest in approaches and architectures that facilitate building such systems. The AQuA architecture is one such approach; its goal is to provide adaptive fault tolerance to CORBA applications by replicating objects. The AQuA archit...

BACKGROUND Aqua-cycling is easy to learn, acceptable, and safe for patients with knee osteoarthritis. It can therefore be an ideal component of aquatic circuit training. OBJECTIVE To investigate the feasibility of a small group-based aquatic exercise programme including aqua-cycling. DESIGN A feasibility study using quantitative (pre-post) and qualitative (cross-sectional) assessments. PA...

Ubiquitäre Umgebungen erfassen meist über Sensoren Informationen über die BenutzerInnen im Raum, analysieren diese Informationen und passen die Umgebungen an die anwesenden Benutzer an. Beispielsweise kann ein Konferenzraum anhand der anwesenden Personen und deren Positionen und Aktionen auf die Art der Sitzung schließen und die Rechnerkonfiguration, die Beleuchtung, die Jalousien, die Projekti...

Decreased permeability to imipenem is the most frequent mechanism of imipenem resistance in Pseudomonas aeruginosa. We have determined the presence of OprD porin protein, an imipenem influx channel, in 70 carbapenem-resistant P. aeruginosa clinical isolates by Western blot analysis using rabbit anti-OprD polyclonal antibody. Ninety-eight percent (54 of 55 isolates) of imipenem-and meropenem-res...

Neisserial porins are strong immune adjuvants and B cell activators. The effect of neisserial porin PorB on activation-induced cell death was investigated, as a potential additional mechanism of the porin's immunopotentiating ability. Neisserial porins interact with target cells to localize intracellularly in the mitochondrial compartment without negatively affecting cellular survival. Pretreat...

Carbapenem-resistance is a great challenge for antimicrobial therapy in Pseudomonas aeruginosa multidrug-resistant infections, as it reduces therapeutic options. This study investigated carbapenem-resistance mechanisms six strains of non-carbapenemase-producing P. aeruginosa. Minimal inhibitory concentrations imipenem and meropenem were determined by epsilometric test broth microdilution. Mutat...