An antibodylike paraprotein has been isolated from a patient with multiple myeloma and autoimmune hyperlipoproteinemia. The paraprotein bound to apolipoprotein B (apo B)-containing lipoproteins that formed macromolecular aggregates, and globules thought to be aggregated complexes of lipoproteins and reactive immunoglobulins were observed circulating within the retinal blood vessels of this pati...

The HepG2 cell line has been used extensively to study the synthesis and secretion of apolipoprotein (apo) B. In this study, we tested whether gene-targeting techniques can be used to inactivate one of the apo B alleles in HepG2 cells by homologous recombination using a transfected gene-targeting vector. Our vector contained exons 1-7 of the apo B gene, in which exon 2 was interrupted by a prom...

Background The significant association between total cholesterol (TC), non-high-density lipoprotein cholesterol (non-HDL), and low-density lipoprotein cholesterol (LDL) has been shown to be associated with Apolipoprotein B (Apo B). The objective of this study was to assess whether abnormal levels of TC, non-HDL and LDL can be used as predictors of abnormal serum Apo B levels. Results TC (r = ...

Apolipoprotein B-100 has a crucial structural role in the formation ofVLDL and LDL. Familial hypobetalipoproteinemia, a syndrome in which the concentration of LDL cholesterol in plasma is abnormally low, can be caused by mutations in the apo B gene that prevent the translation of a full-length apo B-100 molecule. Prior studies have revealed that truncated species of apo B [e.g., apo B-37 (1728 ...

Apolipoprotein (apo) A-IV is primarily associated with HDL or with the lipoprotein-free fraction of plasma, and in small amounts with chylomicrons and VLDL. The aim of the present study was to assess the effect of a fatty meal on the postprandial variation in plasma apo A-IV and on its distribution among lipoprotein fractions following absorption of fat. Twenty healthy male subjects participate...

As genetically determined apolipoprotein E (apo E) phenotypes influence serum cholesterol concentration, we analysed whether serum triglyceride values are also affected by the apo E phenotypes in infants. Non-fasting serum triglyceride values were measured in 7- and 13-month-old participants in the STRIP project, a randomised, prospective trial aimed at reducing children's exposure to known ath...

Apolipoproteins are proteins associated with lipids in lipoprotein particles. They play important roles in lipoprotein metabolism, such as transport of these hydrophobic molecules in plasma aqueous medium, binding to specific receptors in cell surface to correctly direct lipids to target organs and body tissues, and activation or inhibition of enzymes involved in lipid metabolism. Apolipoprotei...

Apolipoproteins (Apo) A-I, A-II, A-IV, and E possess repeated amphipathic helical regions with the same genomic structure and are members of a multigene family that probably evolved from a common ancestral gene [1]. These apolipoproteins play pivotal roles in lipid transport and lipoprotein metabolism, and their carboxyl-terminal domain is critical for lipid binding [2]. The purpose of this com...

A double antibody radioimmunoassay was developed for apolipoprotein A-II (apo A-II), one of the two major apoproteins of human high density lipoproteins (HDL). Apo A-II contains two identical polypeptide chains linked by a disulfide bond. A specific antiserum was raised in sheep. Tracer apo A-II was radiolabeled with 125I by the Bolton-Hunter technique. Reduction and carboxymethylation of apo A...

The interactions of low density lipoprotein (LDL) and apolipoprotein (apo) B-100 segments with chondroitin-6-SO4 rich aortic proteoglycans aggregate (CSPG) were studied by using quantitative frontal elution affinity chromatography. The affinity of the agarose-CSPG was higher for LDL than for very low density lipoprotein and high density lipoprotein was not bound. LDL from different individuals ...