Liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry (LC-APCI-MS) method has been developed for the measurement of the concentrations of 20(S)-ginsenoside Rh1 and its aglycone 20(S)-protopanaxatriol in rat plasma with panaxatriol as internal standard. The method involved single liquid-liquid extraction of both 20(S)-ginsenoside Rh1 and 20(S)-protopanaxatriol from pla...

Triphosgene was decomposed quantitatively to phosgene by chloride ion. The reaction course was monitored by IR spectroscopy (React-IR), showing that diphosgene was an intermediate. The methanolysis of triphosgene in deuterated chloroform, monitored by proton NMR spectroscopy, gave methyl chloroformate and methyl 1,1, 1-trichloromethyl carbonate in about a 1:1 ratio, as primary products. The rea...

Salvinorin A was quantitated in human and rhesus monkey plasma, rhesus monkey cerebrospinal fluid, and human urine by negative ion LC-MS/APCI. The method for Salvinorin A has been fully validated, the LLOQ using FDA guidelines is 2 ng/mL for 0.5 mL plasma samples. The linear range was from 2 to 1000 ng/mL. Several derivatives in the Salvinorin family can also be analyzed by this method; d(3)-Sa...

A series of laboratory experiments on the chemical composition of aerosol particles formed after photodissociation of CH212, a major volatile alkyl halide released from macroalgae, have been performed in a laboratory scale reaction chamber using on-line atmospheric pressure chemical ionization mass spectrometry (APCI/MS). Based on the mass spectrometric results and the molecular properties of i...

An unusual regiospecific diepoxidation of an isomer of C60(CF3)10 was discovered to slowly occur in solution while exposed to air. This is the only known example of a perfluoroalkyl fullerene undergoing epoxidation under ambient conditions. This compound was characterized by a variety of analytical methods including 19F NMR spectroscopy, APCI-MS, UV-vis spectroscopy, and X-ray crystallography. ...

The applicability of Supercritical Fluid Chromatography (SFC) can be substantially enhanced by coupling SFC to Mass Spectrometric detection (MSD). In this Technical Overview, two instrument configurations are described that can be used to connect the Agilent 1260 Infinity Analytical SFC to a mass spectrometer either using an atmospheric pressure chemical ionization (APCI) or an electrospray (ES...

The production area mislabeling of a food product is considered fraudulent practice worldwide. In this work, method that uses ultra-high-performance liquid chromatography coupled to high-resolution mass spectrometry using atmospheric pressure chemical ionization (UHPLC–APCI–HRMS) was used for the geographical origin authentication paprika based on determination capsaicinoids and carotenoids. Sa...

The development of a robust ionization source using the counter-flow APCI, miniature mass spectrometer, and an automated sampling system for detecting explosives are described. These development efforts using mass spectrometry were made in order to improve the efficiencies of on-site detection in areas such as security, environmental, and industrial applications. A development team, including t...

A selective and sensitive method for the simultaneous determination of five avermectins (abamectin, ivermectin, doramectin, emamectin and eprinomectin) and one milbemycin (moxidectin) in porcine liver, bovine meat and fish tissue was developed. The method involved extraction with acetonitrile and purification by C18 solid-phase extraction. Detection was carried out using liquid chromatography c...

Headspace techniques have been extensively employed in food analysis to measure volatile compounds, which play a central role in the perceived quality of food. In this study atmospheric pressure chemical ionisation-mass spectrometry (APCI-MS), coupled with gas chromatography-mass spectrometry (GC-MS), was used to investigate the complex mix of volatile compounds present in Cheddar cheeses of di...