serological methods are commonly used methods for detection of viruses. preparation of pure viral antigens is a crucial step in production of antibodies required for serological studies. in this research the gene encoding coat protein of a beet western yellows virus (bwyv) isolate from iran was amplified by pcr and was ligated into a bacterial expression vector (pet26b) to obtain pet-bwyv-cp cl...

OBJECTIVE The main aims of this study were to explore the molecular structural relationship between Human epididymis protein 4 (HE4) and Lewis y antigen by determining their expression patterns and clinical significance in ovarian epithelial carcinoma. METHODS The structural relationship between HE4 and Lewis y antigen was examined using immunoprecipitation and confocal laser scanning microsc...

Background: Recent studies have found some genetic variants as a risk factor for autoimmune diseases such as Multiple Sclerosis (MS). Cluster of Differentiation 226 (CD226) is one of the risk factors for MS.  Objectives: The present study aimed to evaluate the gene expression of CD226, and its protein serum level in peripheral blood samples of MS patients and healthy individuals. Materials & ...

Background and Aims: Shigellosis is an acute gastroenteritis and Invasion plasmid antigen C (IpaC) is the first effector protein for Shigella invasion of intestinal cells. Among lactic acid bacteria, Bifidobacterium lactis (B. lactis) has received increasing attention for protection of a potential host against gastrointestinal infections. The aim of this study was to investigate the inhibitory ...

Leishmaniasis is a major infectious disease of considerable public health in more than 86 countries around the world. Several approaches toward vaccine development against this disease have been taken. Glycoprotein (gp63) is conserved among diverse species of Leishmania and has induced immunological responses in murine models. Therefore, this glycoprotein has been considered as a second generat...

individual preparation of two human t-cell lymphotropic virus type i (htlv-i) diagnostic gst fused peptides (mta-1 and gd21) is time-consuming and expensive. the aim of this study was to design a novel single chimeric antigen (sca) to obviate separate expression of proteins and reduce the cost of reagent preparation.structural protein fragments, including immunodominant b cell linear epitopes, ...

background : echinococcosis or hydatidosis is a chronic, zoonotic worldwide infection caused by the larval stage of the dog taeniid tapeworm echinococcus granulosus. vaccination has been considered as one of the ways to prevent of hy-datidosis in recent decades. the aim of this study was to construct a pcdna3.1 eukaryotic expression vector contain-ing the subunit 8-kda antigen b (hyd1) of e. gr...

background occult hepatitis b infected (obi) patients cannot eradicate hepatitis b virus (hbv)-dna from their liver and peripheral blood, completely. objectives the main aim of this study was to investigate the rate of hla-a2 expression on peripheral blood mononuclear cells (pbmcs) of patients with obi. materials and methods in this experimental study, intensity of hla-a2 was measured on the pb...