Two human erythroleukemia cell lines, HEL and KS62, express transglutaminase activity. The enzyme was identified as a tissue transglutaminase following Chromatographie purification. All-fra/w-retinoic acid (10 MM)stimulated differentiation in HEL cells as judged by a 4-fold increase in hemoglobin content and a reduction in cell proliferation. The transglutaminase activity increased 9-fold. This...

CONTEXT Celiac disease, one of the best-known autoimmune human leukocyte antigen-dependent disorders, has a relatively increased prevalence in first-degree relatives. OBJECTIVE To determine the prevalence of celiac disease in siblings of patients with confirmed celiac disease. METHODS Siblings of confirmed celiac disease patients in our center were identified and enrolled in this study. The...

Tissue transglutaminase functions as a protein crosslinking enzyme and an integrin-binding adhesion co-receptor for fibronectin on the cell surface. These activities of transglutaminase and the involvement of this protein in cell-matrix adhesion, integrin-mediated signaling, cell migration and matrix organization suggest a precise and efficient control of its cell-surface expression. We report ...

Transglutaminases are a superfamily of isoenzymes found in cells and plasma. These enzymes catalyze the formation of ε-N-(γ-glutamyl)-lysyl crosslinks between proteins. Cystamine blocks transglutaminase activity and is used in vitro in human samples and in vivo in mice and rats in studies of coagulation, immune dysfunction, and inflammatory disease. These studies have suggested cystamine blocks...

Background and aims: Coeliac disease (CD) is a multifactorial disorder which has an autoimmune component characterised by the occurrence of disease specific autoreactive antibodies against the enzyme tissue transglutaminase (tTG). The aim of this study was to investigate whether binding of antibodies to the enzyme influences tTG activity. Methods: tTG activity was assayed in the presence of imm...

Phospholipases A2 are enzymes that liberate membrane-bound lipids in a tissue and cell-specific fashion. Group V secretory phospholipase A2 is necessary for the development of M2 macrophages and their effector functions in a mouse model of the T-helper-2 allergic airway inflammation. However, the function of group V phospholipase A2 in human M2 activation and T-helper-2 inflammation is ill-defi...

version of a paper published in Clinical and experimental immunology. This paper has been peer-reviewed but does not include the final publisher proof-corrections or journal pagination. "Reduction of tissue transglutaminase autoantibody levels by gluten-free diet is associated with changes in subsets of peripheral blood lymphocytes in children with newly diagnosed coeliac disease" Reduction of ...

BACKGROUND Serologic methods have been used widely to test for celiac disease and have gained importance in diagnostic definition and in new epidemiologic findings. However, there is no standardization, and there are no reference protocols and materials. METHODS The European working group on Serological Screening for Celiac Disease has defined robust noncommercial test protocols for immunoglo...

S FORMING THE BASIS OF THIS WORK 1. Sárdy M, Kárpáti S. Comparison of a tissue transglutaminase enzyme-linked immunosorbent assay with the endomysium antibody test in the diagnosis of dermatitis herpetiformis. J Invest Dermatol 1999; 113:448. IF: 4.903 2. Preisz K, Kárpáti S, Sárdy M, Zágoni T, Horváth A. Codistribution of tissue trans-glutaminase and tissue-bound IgA in jejunum of patients wit...