نتایج جستجو برای: anti peroxidase antibody

تعداد نتایج: 502114  

Journal: :The Analyst 2000
Q Z Zhu H H Yang D H Li Q Y Chen J G Xu

Iron tetrasulfonatophthalocyanine (FeTSPc), a peroxidase mimic, was used as a labeling reagent and poly(N-isopropylacrylamide) (PNIP) as the separation support of the immune complex for the mimetic-enzymatic immunoassay of hepatitis B surface antigen (HBsAg). PNIP was precipitated from aqueous solution when the ambient temperature was higher than its lower critical solution temperature of 31 de...

F Motamedi-Sedeh , M Shamsi-Shahrabadi ,

Background and Aims: Rotaviruses are one of the main causative agents of acute viral gastroenteritis in both young human and animals. Several methods have been used for diagnosis of rotavirus infection. Most of these methods are not easily available in Iran. Methods: In our study attempt was made to develop a method which could replace the commercially imported Kit. Rota virus was purified and...

حبیب زاده عمران, وحید, رستگارپور, هادی, فتاحی, وحید, مصطفی زاده, امراله, میترا علمی, مریم, نوری, حمید رضا,

Background and objectives: Anti-human immunoglobulin G (IgG) antibody is produced against human IgG in various laboratory animals. The present study tried not only to produce anti-human IgG, but also to assess different antigen injection techniques leading to optimal production of anti-human globulin.Methods: The antibody was separated from human serum using precipitation method with sodium sul...

Journal: :iranian journal of medical sciences 0
a. behzad-behbahani a. mafi-nejad s.z. tabei k.b. lankarani m. rashidi m. rasouli

background: in spite of available, and sensitive screening assay for detection of hepatitis b virus surface antigen (hbsag), occasional cases of post-transfusion hepatitis b virus infection are still observed. the aim of the present study was to assess the prevalence of positive anti hepatitis b core (anti-hbc) and presence of hbv-dna in serum sample of healthy blood donors negative for both hb...

Journal: :Clinical science 1997
R S McIntosh M S Asghar A P Weetman

1. The analysis of the antibody response in autoimmune thyroid disease has followed several historical trends. It was the investigation of thyroid-reactive antibody that allowed the initial characterization of the three principle thyroid autoantigens, thyroglobulin, thyroid peroxidase and the thyroid stimulating hormone receptor. 2. Analysis can be grouped under two broad areas: analysis of the...

A. Behzad-Behbahani A. Mafi-Nejad A. Torab A.R. Salah B. Pourabbas K.B. Lankarani M. Rashidi M. Rasouli S.Z. Tabei

Background: In spite of available, and sensitive screening assay for detection of hepatitis B virus surface antigen (HBsAg), occasional cases of post-transfusion hepatitis B virus infection are still observed. The aim of the present study was to assess the prevalence of positive anti hepatitis B core (anti-HBc) and presence of HBV-DNA in serum sample of healthy blood donors negative for both HB...

Journal: :iranian journal of immunology 0
mohammad fereidouni department of immunology, birjand university of medical sciences, birjand, iran farhzad jabbari azad immunology research center, mashhad university of medical sciences, mashhad, iran mahmoud mahmoudi immunology research center, mashhad university of medical sciences, mashhad, iran abdolreza varasteh immunology research center, mashhad university of medical sciences, mashhad, iran reza farid hosseini department of allergy and immunology, ghaem hospital, mashhad, iran

background: invariant natural killer cells (inkt) are an important immunoregulatory t cell subset. currently several flow cytometry-based approaches exist for the identifi-cation of inkt cells, which rely on using the 6b11 monoclonal antibody or a combina-tion of anti-vα24 and anti-vβ11 antibodies. objective: the aim of this study was to compare the ability of two flow cytometry-based methods f...

Journal: :BioTechniques 1996
S K Lee S L Yu H Alexander S Alexander

Goat anti-mouse immunoglobulin horseradish peroxidase (HRP) conjugate was diluted in PBS containing 10% BSA. This conjugate has been successfully used from various manufacturers; however, specific lots need to be titrated for optimal performance. One particular preparation of HRPconjugated goat anti-mouse IgG, IgM and IgA (Catalog No. A 0412; Sigma Chemical, St. Louis, MO, USA) was diluted at 1...

E. Jalali M. Ghorbanpoor, M. R. Seyfiabad Shapouri S. Goraninejad

Definitive diagnosis of brucellosis is made by isolation of the causative agents, which is a timeconsumingprocedure. To evaluate the efficacy of Dot-ELISA for detecting brucellae in clinical samples, 94different specimens taken from animal origin were cultured on brucella selective culture media and colonieswere identified biochemically. The specimens were also examined after centrifugation by ...

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