The widespread use of triclosan in personal care products as an antimicrobial agent is leading to its alarming tissue-bioaccumulation including human brain. However, knowledge potential effects on the vertebrate nervous system still limited. Here, we hypothesized that sublethal concentrations are potent enough alter motor neuron structure and function zebrafish embryos exposed for prolonged dur...

SUMMARY To fully understand animal transcription networks, it is essential to accurately measure the spatial and temporal expression patterns of transcription factors and their targets. We describe a registration technique that takes image-based data from hundreds of Drosophila blastoderm embryos, each co-stained for a reference gene and one of a set of genes of interest, and builds a model Vir...

We report the cloning and functional characterization of the first heat-shock transcription factor that is specifically expressed during embryogenesis in the absence of environmental stress. In sunflower embryos this factor, HaHSFA9, trans-activated promoters with poor consensus heat-shock cis-elements, including that of the seed-specific Hahsp17.6G1 gene. Mutations that improved the heat-shock...

The maternal-to-zygotic transition (MZT) is a process that occurs in animal embryos at the earliest developmental stages, during which maternally deposited mRNAs and other molecules are degraded and replaced by products of the zygotic genome. The zygotic genome is not activated immediately upon fertilization, and in the pre-MZT embryo post-transcriptional control by RNA-binding proteins (RBPs) ...

Cellular differentiation during embryogenesis involves complex gene regulation to enable the activation and repression of genes. Here, we show that mesodermal competence is inhibited in Xenopus embryos depleted of histones H3 and H3.3, which fail to respond to Nodal/Activin signaling and exhibit concomitant loss of mesodermal gene expression. We find that transcriptional activation in gastrula ...

To fully understand animal transcription networks, it is essential to accurately measure the spatial and temporal expression patterns of transcription factors and their targets. We describe a registration technique that takes image-based data from hundreds of Drosophila blastoderm embryos, each costained for a reference gene and one of a set of genes of interest, and builds a model VirtualEmbry...

during spring, summer and winter seasons, sistani donor cows, with normal reproductive status, were superovulated and embryos were recovered non-surgically on day 7. grade a blastocyst embryos were either transferred fresh (spring) or frozen (summer and winter). recovered embryos during summer and winter were exposed to glycerol and frozen using conventional method. during spring season, recipi...

Hatching plasticity occurs in response to a wide range of stimuli across many animal taxa, including annelids, arthropods, mollusks, and chordates. Despite the prominence of echinoderms in developmental biology and more than 100 years of detailed examination of their development under a variety of conditions, environmentally cued hatching plasticity has never been reported in the phylum Echinod...

We explored the amount and timing of temporal synchrony necessary to facilitate prenatal perceptual learning using an animal model, the bobwhite quail. Quail embryos were exposed to various audiovisual combinations of a bob-white maternal call paired with patterned light during the late stages of prenatal development and were tested postnatally for evidence of prenatal auditory learning of the ...

BMJ | 8 SepteMBer 2007 | VoluMe 335 Zosia Kmietowicz london The Human Fertilisation and Embryology Authority, the United Kingdom’s fertility watchdog, will decide this week whether to approve in principle the use of hybrid animal-human embryos for research. Results of a public consultation on the matter, which were released before the authority’s decisive meeting that was due to be held on Wedn...