We have purified approximately 6600-fold an approximately 40-kDa protein (Ter protein) encoded by Escherichia coli that specifically binds to two sites at the 216-base-pair replication terminus (tau) of the plasmid R6K. Chemical footprinting experiments have shown that the Ter protein binds to two 14- to 16-base-pair sequences that exist as inverted repeats in the tau fragment. Site-directed mu...

Introduction: The evolution of third and fourth generation linked, semi-constrained total elbow replacements (TER) have employed an increasing use of modularity to facilitate component linkage and inter-operative flexibility. In general, modular connections (MC) used in orthopedic devices may carry inherent risks of adverse events resulting from component fracture, loosening, micromotion, frett...

We examine a new, intuitive measure for evaluating machine-translation output that avoids the knowledge intensiveness of more meaning-based approaches, and the labor-intensiveness of human judgments. Translation Edit Rate (TER) measures the amount of editing that a human would have to perform to change a system output so it exactly matches a reference translation. We show that the single-refere...

Following TER nets, an approach to the modelling of time in high-level Petri nets, we propose a model of time within (attributed) graph transformation systems where logical clocks are represented as distinguished node attributes. Corresponding axioms for the time model in TER nets are generalised to graph transformation systems and semantic variations are discussed. They are summarised by a gen...

In this paper, the problem of speech enhancemen t when only corrupted speech signal is available for processing is considered. For this , the Kalman f i l ter ing method is s tudied and compared with t e Wiener f i l ter ing method. Its performance is found to be signif ican t ly be t te r han the Wiener f i l ter ing method. A delayed-Kalman fi l tering method is also proposed which improves t...

RESUMO Este artigo constitui um desdobramento da reflexão sobre a Hermenêutica enquanto ética tecida em torno cunhagem, descrição e fundamentação expressão Eu Hermeneuticus, que trata o outro como fim si mesmo não objeto. Aqui, sob égide do pressuposto gadameriano segundo qual “o somente tem também direito, mas talvez pode ter razão”, pretendo evidenciar justificar centralidade alteridade na te...

this study investigated intra-specific variation in the growth parameters and dry mat-ter partitioning of sugar beet cultivars at different levels of salinity (0, 50, 150, 250 and 350 mm nacl + cacl2 in 5:1 molar ratio) using four cvs, one british (madison) and three iranian cvs (7233-p12, 7233-p21 and 7233-p29). the plants were grown in a controlled greenhouse environment for about 18 weeks. a...

Termination of replication forks at the natural termini of the rDNA of Saccharomyces cerevisiae is controlled in a sequence-specific and polar mode by the interaction of the Fob1p replication terminator protein with the tandem Ter sites located in the nontranscribed spacers. Here we show, by both 2D gel analyses and chromatin immunoprecipitations (ChIP), that there exists a second level of glob...