Several published reports have been devoted to insecticide resistance in Anopheles gambiae in Africa. However, there are still not enough published reports about the impact of insecticide resistance on malaria transmission. In this study, we proposed to investigate the presence of the circum-sporozoite antigen of Plasmodium falciparum in A. gambiae carrying kdr and Ace-1 genes. A. gambiae (3,11...

Knockdown resistance ( kdr) and CYP9K1 genotypes were detected by a MOLDI-TOF based SNP genotyping assay (Sequenom iPLEX) in samples of Anopheles gambiae collected at 13 sites throughout the Union of the Comoros and Dar es Salaam, Tanzania during February and March 2011. All A. gambiae specimens collected in the Comoros were homozygous for the susceptible kdr alleles (+/+) while 96% of A. gambi...

) and genotypes were detected by a Knockdown resistance kdr CYP9K1 MOLDI-TOF based SNP genotyping assay (Sequenom iPLEX) in samples of collected at 13 sites throughout the Union of the Comoros Anopheles gambiae and Dar es Salaam, Tanzania during February and March 2011. All . A gambiae specimens collected in the Comoros were homozygous for the susceptible kdr alleles (+/+) while 96% of from Dar...

Background: Insect symbionts have the potential to block the transmission of vector-borne diseases by their hosts. The advancement of a symbiont-based transmission blocking strategy for malaria requires the identification and study of Anopheles symbionts. Methods: High throughput 16S amplicon sequencing was used to profile the bacteria associated with Anopheles gambiae sensu lato and identify p...

"Far-West" Africa is known to be a secondary contact zone between the two major malaria vectors Anopheles coluzzii and A. gambiae. We investigated gene-flow and potentially adaptive introgression between these species along a west-to-east transect in Guinea Bissau, the putative core of this hybrid zone. To evaluate the extent and direction of gene flow, we genotyped site 702 in Intron-1 of the ...

BACKGROUND Rapid demographic growth in Douala city, Cameroon, has resulted in profound ecological and environmental changes. Although demographic changes can affect anopheline mosquito breeding sites, there is a lack of understanding about the epidemiological impact that such changes might have on vector ecology and malaria transmission. METHODS A 12-month entomological study was conducted in...

In our recent review on genetic and physical mapping in mosquitoes, we discussed the future of mosquito genomics (Severson et al., 2001). We stated that reagents and methodologies were in place for fine-scale mapping in Culicidae and for map-based positional cloning in Aedes aegypti and Anopheles gambiae. We reported that an An. gambiae genome project had been initiated and predicted that there...

BACKGROUND & OBJECTIVES A study was conducted to characterise larval habitats and to determine spatial heterogeneity of the Anopheles mosquito larvae. The study was conducted from May to June 1999 in nine villages along the Kenyan coast. METHODS Aquatic habitats were sampled by use of standard dipping technique. The habitats were characterised based on size, pH, distance to the nearest house,...

The glutamate-gated chloride channel (GluCl) is a highly sensitive insecticide target of the avermectin class of insecticides. As an alternative to using chemical insecticides to kill mosquitoes, we tested the effects of purified immunoglobulin G (IgG) targeting the extracellular domain of GluCl from Anopheles gambiae (AgGluCl) on the survivorship of three key mosquito disease vectors: Anophele...

In 13 villages in the savannah zone and 21 villages in the forest zone of Côte d'Ivoire, the biting density of the principal malaria vector, Anopheles gambiae, was studied as a function of rice cultivation in the inland valleys in a 2-km radius around each village. In the savannah villages, during the main season cropping period, surface water on rice-cultivated and to a lesser extent on uncult...