The anti-diabetic potential of whole unripe jackfruit (peel with pulp, flake, and seed) was investigated using inhibitory assays for α-glucosidase, α-amylase, aldose reductase, glycation at multiple stages. Using activity-guided repeated fractionation on a silica gel column chromatography, dietary flavonoid rutin potent antihyperglycemic activity extracted from the methanol extract flour (MJ). ...

Chen, Z.-Y., Brown, R. L., Russin, J. S., Lax, A. R., and Cleveland, T. E. 1999. A corn trypsin inhibitor with antifungal activity inhibits Aspergillus flavus α-amylase. Phytopathology 89:902-907. In this study, we found that the inhibition of fungal growth in potato dextrose broth (PDB) medium by the 14-kDa corn trypsin inhibitor (TI) protein, previously found to be associated with host resist...

In this study, the antidiabetic potential of aqueous extract Origanum minutiflorum was tested using streptozotocin induced diabetic rats. Changes in body weight, blood glucose level, biochemical parameters, antioxidant enzyme activities and histopathological examination kidney were evaluated. The administration carvacrol to group rats caused significant decrease glucose, parameters serum signif...

Pharmacognosy Research,2021,13,3,140-148.DOI:10.5530/pres.13.3.6Published:June 2021Type:Original ArticleAuthors:Basanta Kumar Sapkota, Karan Khadayat, Bikash Adhikari, Darbin Poudel, Purushottam Niraula, Prakriti Budhathoki, Babita Aryal, Kusum Basnet, Mandira Ghimire, Rishab Marahatha, and Niranjan Parajuli Author(s) affiliations:Basanta Sapkota1,2, Khadayat1, Adhikari1, Poudel1, Niraula1, Bud...

Valienamine (1) i s the unsaturated derivative of carbocyclic analogues of glycosylamines. I ts αand β-anomeric i somers ar e n ot i nterconvertible each o ther, existing as chemically stable forms (Fig. 1). Compound 1 was first isolated from antibiotic v alidamycins a nd f ound t o pos sess a s trong i nhibitory a ctivity a gainst α-glucosidase. It was also shown as one of the components of ps...

Starch is a major energy source for all domains of life. Recent advances in structures of starch-degrading enzymes encompass the substrate complex of starch debranching enzyme, the function of surface binding sites in plant isoamylase, details on individual steps in the mechanism of plant disproportionating enzyme and a self-stabilised conformation of amylose accommodated in the active site of ...

Acarbose is a well-known inhibitor of α-glucosidases, α-amylases, cyclomaltodextrin glucanyltransferase (CGTase), and dextransucrase. Bacillus stearothermophilus maltogenic amylase (BSMA) was found to hydrolyze acarbose to give acarviosine-glucose plus glucose and to carry out a transglycosylation reaction to give isoacarbose. Acarviosine-glucose was a potent inhibitor of baker’s yeast α-glucos...

alpha-amylase e.c.3.2.1.1. (1,4 glucan, 4 glucanohydrolase) can be obtained from salivary glands, pancreas and microorganisms such as pseudomonas and asperigllus, as well as muscles and ovarian tubes.1.2 alpha-amylase from bacillus subtilis #1024(a tcc 465)* was purified with a highest degree of purity in our laboratory (31.59 u/mg). the extracellular alpha-amylase was subjected to different pu...