abstract because of relation between digestive enzyme activity and feeding of insects that directly is in relation with the damage of insects; studying digestive enzyme systems is important in finding new control methods based on inhibiting digestive enzyme activity. recently using transgenic plants containing enzyme inhibitors is important in improving resistance of crops to pests because of s...

The cloning of a-amylase gene of S . occidentalis and the construction of starch digestible strain of yeast, S . cerevisiae AS. 2.1364 with ethanol-tolerance and without auxotrophic markers used in fermentation industry were studied. The yeast/E . coli shuttle plasmid YCEpl partial library of S. occidentalis DNA was constructed and a-amylase gene was screened in S. cerevisiae by amylolytic acti...

The presence of multiple forms of alpha-amylase in gibberellic acid-treated embryoless barley half-seeds was demonstrated by separation on diethylaminoethyl-Sephadex and isoelectric focusing polyacrylamide gel disc electrophoresis. Two major alpha-amylase fractions (A and B), each consisting of two to three isozyme components, were purified. alpha-Amylase fractions A and B were distinguishable ...

The present review assesses the potential of the Phaseolus vulgaris a-amylase inhibitor isoform 1 (a-AI1) starch blockers as a widely used remedy against obesity and diabetes. Consumption of the a-amylase inhibitor causes marginal intraluminal a-amylase activity facilitated by the inhibitor’s appropriate structural, physico-chemical and functional properties. As a result there is decreased post...

Amylase is one of the most important enzymes in the world due to its wide application in various industries and biotechnological processes. In this study, amylase enzyme from Hylocereus polyrhizus was encapsulated for the first time in an Arabic gum-chitosan matrix using freeze drying. The encapsulated amylase retained complete biocatalytic activity and exhibited a shift in the optimum temperat...

We describe a column-chromatographic method for measuring amylase activity in cyst fluids of serous ovarian tumors. Using this technique, we confirm and extend the previous report of an "acidic" amylase in serous ovarian tumor cyst fluids. This form of the enzyme was eluted from DEAE-Sephadex mini-columns with a high-salt buffer. It accounted for 45 to 100% of the amylase in 13 cyst fluids. Thi...

Sulfolobus solfataricus secretes an acid-resistant alpha-amylase (amyA) during growth on starch as the sole carbon and energy source. Synthesis of this activity is subject to catabolite repression. To better understand alpha-amylase function and regulation, the structural gene was identified and disrupted and the resulting mutant was characterized. Internal alpha-amylase peptide sequences obtai...

The use of enzymes in feedlot diets has not been evaluated extensively, and most research with high-concentrate diets has been with barley. Beauchemin et al. (1997) evaluated the addition of two enzyme mixtures that varied in the proportion of xylanase and cellulase activity to 95.1% concentrate feedlot diets based on either barley or corn. Feed efficiency for the overall finishing period was i...

The age-dependent development of alpha-amylase expression in utero and during the first two years of life is reported. Separation of salivary and pancreatic amylase isozymes in a discontinuous buffered sheet polyacrylamide electrophoretic system, with subsequent densitometry, provides a reliable semiquantitative method of estimating the proportions of salivary and pancreatic amylases in urine a...