Sequence databases could be efficiently exploited for development of DNA markers if it were known which gene regions reveal the most polymorphism when amplified by PCR. We developed PCR primer pairs that target specific regions of previously sequenced genes from Avena and Zea species. Primers were targeted to amplify 40 introns, 24 exons, and 23 promoter regions within 54 maize genes. We survey...

Safflower is a self-pollinating oilseed crop and has been cultivated since antiquity in the Middle East. become minor crop, to increase its cultivation it necessary characterize introduce new germplasm. Thirty-eight safflower cultivars breeding lines from different countries have screened with 8 AFLP primer combinations assess genetic diversity relationships among these genotypes. primers produ...

Genetic stability of Morus alba, Morus indica, Morus laevigata (indigenous collection) and Morus species (exotic collection) have been studied in in vitro regenerated plants of mulberry (fresh, before and after cryopreservation) using random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers. This study examined the genetic stability of cryopreserved dormant buds o...

The present investigation was undertaken to examine the genetic divergence in 50 mungbean germplasm lines for 13 characters using Mahalanobis D statistics. The genotypes grouped into eight clusters. Cluster VII had maximum intra-cluster distance while inter-cluster distance was highest between clusters V and VII. Cluster means indicated that none of the clusters was superior for all the charact...

Jatropha curcas L. is a rapidly emerging biofuel crop attracting a lot of interest, triggering large investments and rapid expansion of cultivation areas. In the present investigation, the genetic relationships of 29 J. curcas accessions were assessed based on randomly amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) analyses. A total of 72 polymorphic primers (47 RAPD a...

out of fifty-two iranian nonaflatoxigenic strains of aspergillus flavus, which were collected from various substrates (soil and kernel) and sources (peanut, corn and pistachio), fifteen representatives were selected according to their different geographical origins (six provinces: guilan and golestan, ardebil, fars, kerman and semnan) and vegetative compatibility groups (vcgs, ir1 to ir15) for ...

pomegranate is an importantly popular fruit crop with iran the main center of its diversity throghout the world. the richest pomegranate genetic resources believed to exist in iran. the present investigation was carried out to study the genetic diversity of 15 genotypes of pomegranate in tarume zanjan making use of rapd markers. genomic dna was extracted from the plant,s leaf, and tested for th...