OBJECTIVE To report a new grading system and surgical outcome by sealing the gap between the conjunctiva and Tenon capsule. METHODS A total of 32 eyes of 30 patients with pterygia were managed at the Ocular Surface Center from January 1, 2002, through December 31, 2010. The eyes were consecutively operated on by recession; sealing of the gap; covering of exposed medial rectus muscle by amniot...

Human amniotic epithelial cells (HAEC) are formed from epiblasts on the 8th day after fertilization. Because they lack major histocompatibility complex (MHC) antigen, human amniotic tissue transplantation has been used for allotranplantation to treat patients with lysosomal diseases. We have provided evidence that HAEC have multiple functions such as synthesis and release of acetylcholine (ACh)...

purpose: to evaluate the role of amniotic membrane transplantation (amt) in acute ocular chemical burns. methods: patients with grade ii-iv (gii-giv) ocular chemical burns (roper-hall classification) of less than two weeks’ duration underwent amt in addition to medical therapy. patients were followed for at least 6 months and evaluated for ocular pain, visual acuity, epithelial defect healing, ...

AIM Treatment of recurrent pterygium associated with symblepharon requires both suppression of fibrosis and reconstruction of limbal barrier. To achieve this, human amniotic membrane was transplanted and limbal autografts performed. METHODS Four patients with severe symblepharon resulting from multiple surgeries for pterygium were treated. Human amniotic membrane was obtained at caesarean sec...

Vocal fold scars and sulcus cause severe vocal problems, but no optimal treatment has been established. In cases, in which the entire lamina propria is occupied by fibrosis vibratory function completely lost, more powerful regeneration materials should be used, or mucosa totally replaced. Amniotic membrane a scaffold material that rich stem cells growth factors high regenerative effect. It can ...

AIM/PURPOSE OF THE STUDY To develop a one-week storage method, without serum and xenobiotics, that would maintain cell viability, morphology, and phenotype of cultured human limbal epithelial sheets. MATERIALS AND METHODS Human limbal explants were cultured on intact human amniotic membranes for two weeks. The sheets were stored in a hermetically sealed container at 23°C in either a serum-fre...

Injection of amniotic fluid stem cells ameliorates the acute phase of acute tubular necrosis in animals by promoting proliferation of injured tubular cells and decreasing apoptosis, but whether these stem cells could be of benefit in CKD is unknown. Here, we used a mouse model of Alport syndrome, Col4a5(-/-) mice, to determine whether amniotic fluid stem cells could modify the course of progres...

background: burns are among the most common traumas in developing countries, which consume large amounts of medical resources. it is important to find an appropriate material for dressing of burn wounds that improves healing and is readily available, easily applicable, and economical.   methods: in a single-blind randomized controlled clinical trial from march to october 2006, 211 patients with...