A systematic approach was used to evaluate the electrospray ionization mass spectral (ESI-MS) analysis of sucrose octasulfate (SOS), an important pharmaceutical agent. SOS represents a model for other suffated carbohydrates, such as heparin and glycosaminoglycan-derived oligosaccharides that also are highly sulfated and pose difficult analytical problems. A survey of ammonium counterions showed...

A non-saccharified high stress corn mash system was used to study the acceleration of glycolysis in glucose ethanol fermentation by Saccharoniyces cerevisiae. It was found that the controlled addition of ammonium sulfate or ammonium chloride resulted in an accelerated glycolytic rate, independent of the final ethanol yield. Adding high levels of ammonium salt, however, resulted in decreased ext...

The hygroscopic phase transitions and growth factors of mixed particles having as components ammonium sulfate and secondary organic material (SOM) were measured. The SOM was generated by the dark ozonolysis of α-pinene, and organic particle mass concentrations of 1.63 and 12.2 μg m−3 were studied. The hygroscopic properties were investigated using a 1×3 tandem differential mobility analyzer (1×...

Studies were undertaken to find a substance or substances for use in primary binding types of tests to detect humoral antibodies in rabbits and monkeys exposed to the tubercle bacillus that would distinguish between strains of mycobacteria. The antigen employed was a component of the 5159 strain of Mycobacterium tuberculosis that was obtained following sonification, ultracentrifugation, electro...

Antigen-specific factors produced by the T-cell growth factor-dependent T-cell continuous line E-9M(+) were partially purified. Gel analysis of the twice-affinity-purified eluate of a poly(Tyr,Glu)-poly(DLAla)--poly(LLys) [(T,G)-A--L] column revealed the existence of iodinated bands with molecular weights of 17,000 and 15,000, in addition to a diffuse band of high molecular weight. The specific...

The procedures for culturing the experimental organism, P. jhorescens, and for preparing the crude cell-free extract have been described previously (1). Partial Purification of Crude Enzyme Preparation-Powdered ammonium sulfate was added to a given volume of the crude cell-free extract to obtain 20 per cent saturation. The resulting precipitate was collected by centrifugation for 30 minutes at ...

This paper describes a method for the selective precipitation and purification of a monovalent protein (carbonic anhydrase is used as a demonstration) from cellular lysate using ammonium sulfate and oligovalent ligands. The oligovalent ligands induce the formation of protein-ligand aggregates, and at an appropriate concentration of dissolved ammonium sulfate, these complexes precipitate. The pu...

Production of human proteins in Pichia pastoris has significant advantages. However, there is still need for improvement in various stages of its downstream processing like clarification and purification. In fact downstream processes are usually the most critical part of production of biotech products. This work aimed to evaluate the effect of two steps added to the downstream processes ...

BACKGROUND & OBJECTIVES Studies were conducted to determine the effect of ammonium sulfate (AM) and muriate of potash (MOP) fertilizers on survival and development of immature stages of Culex quinquefasciatus Say, a major vector of Bancroftian filariasis in Africa. METHODS Twenty I instar larvae each were added in four doses of each fertilizer dissolved in one litre of deionised water and in ...

INTRODUCTION  Fibrin tissue adhesive, which has applications in several areas of medicine, can be prepared by different methods. AIM  To compare fibrin tissue adhesives prepared by 3 different methods. METHOD  In this prospective experimental laboratory study, fibrin tissue adhesives prepared by the use of plasma fibrinogen (group 1), cryoprecipitation (group 2), and precipitation by ammoni...