Fe(II)/2OG-dependent dioxygenases of the AlkB family catalyze a direct removal alkylated damages in course DNA and RNA repair. A human homolog E. coli ALKBH3 protein is able to hydroxylate N1-methyladenine, N3-methylcytosine, N1-methylguanine single-stranded RNA. Due its contribution an antitumor drug resistance, this enzyme considered promising therapeutic target. The elucidation ALKBH3’s stru...

Gas-liquid mass transfer of gaseous reactants is a major limitation for high space-time yields, especially for O2 -dependent (bio)catalytic reactions in aqueous solutions. Herein, oxygenic photosynthesis was used for homogeneous O2 supply via in situ generation in the liquid phase to overcome this limitation. The phototrophic cyanobacterium Synechocystis sp. PCC6803 was engineered to synthesize...

Mammals have nine homologues of the Escherichia coli AlkB repair protein: Alkbh1-8, and the fat mass and obesity associated protein FTO. In this report, we describe the first functional characterization of mouse Alkbh7. We show that the Alkbh7 protein is located in the mitochondrial matrix and that an Alkbh7 deletion dramatically increases body weight and body fat. Our data indicate that Alkbh7...

DNA can be damaged by various intracellular and environmental alkylating agents to produce alkylation base lesions. These base damages, if not repaired promptly, may cause genetic changes that lead to diseases such as cancer. Recently, it was discovered that some of the alkylation DNA base damage can be directly removed by a family of proteins called the AlkB proteins that utilize a mononuclear...

Driven by oncogenic signaling, glutamine addiction exhibited by cancer cells often leads to severe glutamine depletion in solid tumors. Despite this nutritional environment that tumor cells often experience, the effect of glutamine deficiency on cellular responses to DNA damage and chemotherapeutic treatment remains unclear. Here, we show that glutamine deficiency, through the reduction of alph...

Abstract Neutrophil generation and effective recruitment into the site of infection are essential for innate immune defense against bacterial infection. These processes that directed by extracellular signals such as growth factors or chemokines have been extensively studied, yet intrinsic new mechanism determining neutrophil needs further investigation. N6-methyladenosine (m 6A) RNA modificatio...

Sewage sludge digestate is a valuable organic waste which can be used as fertilizer in soil bioremediation. not only good source of nutrients but also rich bacteria carrying alkB genes, are involved aliphatic hydrocarbons metabolism. Increase genes ratio polluted soils has been observed to improve bioremediation efficiency. In this study, for the first time, genetic potential indigenous microor...