Trisodium phosphate (TSP) has been reported to decrease the recovery of salmonellae from processed poultry carcasses. It has been suggested that the high pH and detergent-like properties of TSP solutions are responsible for the reduction in salmonellae recovery. This project was conducted to determine if controlling pH during salmonellae pre-enrichment alters the effect of TSP on salmonellae re...

Treatment of the BeWo line of choriocarcinoma cells with methotrexate in doses that inhibit DNA synthesis causes a tenfold increase in synthesis of human chorionic gonadotropin and a threefold increase in activity of placental alkaline phosphatase. No concomitant increase in lactic dehydrogenase activity occurs under these conditions. This effect of methotrexate can be blocked by simultaneous a...

Broken rice is an underutilized by-product of milling. Proteins prepared from broken rice by treatments with alkaline protease and papain have been characterized with regard to nutritional and functional properties. The protein content and the protein recovery were 56.45 and 75.45 % for alkaline protease treatment, and 65.45 and 46.32 % for papain treatment, respectively. Protease treatment inc...

A cell surface-localized glycoprotein that exhibits alkaline phosphatase activity was induced by treatment of mouse L-cell cultures with dibutyryl cyclic AMP. Treatment of cells with 1.5 mM dibutyryl cyclic AMP for a period of 7 days resulted in a approximately 2000-fold increase in the specific activity of the enzyme. Enzyme induction was dependent upon de novo RNA and protein biosynthesis sin...

The effects of pre-treating different types of roughages with alkali on the efficacy of exogenous fibrolytic enzymes for improving their digestibility were studied in vitroin factorial arrangement 4 × 3 × 5 (enzyme, treatment and roughage types). Two fibrolytic feed enzymes novozyme (N) and celloclast (C) their combination (N+C) were evaluated for their potential to improve in vitrodegradation ...

The large-conductance, calcium-activated BK-α/β4 potassium channel, localized to the intercalated cells of the distal nephron, mediates potassium secretion during high-potassium, alkaline diets. Here we determine whether BK-α/β4-mediated potassium transport is dependent on epithelial sodium channel (ENaC)-mediated sodium reabsorption. We maximized sodium-potassium exchange in the distal nephron...

The present study evaluated the basal DNA damage and the cellular response to this damage induced by in vitro administration of Etoposide in lymphocytes donated by twenty untreated breast cancer (BC) patients and twenty age-matched healthy women. Micronucleus (MN) and alkaline Comet assays were performed in cultured peripheral blood lymphocytes (PBL) according to a standard protocol for in vitr...