Background: Enzymes are the biological catalysts which accelerate the various cellular reactions. In general, the enzymes alkaline phosphatase (ALPase), acid phosphatase (ACPase), 5′-nucleotidase (5′-Nu) and glucose-6phosphatase (G-6-Pase) have important roles in metabolism. In the present study, a comparative account of the localization of activity of four enzymes acid phosphatase, alkaline ph...

In the kidney of the adult mouse the tubular epithelium in the most proximal (Pi) segment, corresponding roughly to the convoluted portion of the proximal tubule, shows strong alkaline phosphatase activity, and the brush border is moderately reactive to the periodic acid Schiff (PAS) method. In the more distal (P2) segment of the proximal tubule there is no alkaline phosphatase activity, and th...

We have examined the extracellular phosphatases produced by the terrestrial green alga Chlamydomonas reinhardtii in response to phosphorus deprivation. Phosphorus-deprived cells increase extra-cellular alkaline phosphatase activity 300-fold relative to unstarved cells. The alkaline phosphatases are released into the medium by cell-wall-deficient strains and by wild-type cells after treatment wi...

Serum alkaline phosphatase (SAP) rises after fat ingestion in humans (Langman et al., 1966; Warnock, 1968) and in rats (Jackson, 1952). Recent work in this unit has provided immunological evidence that, in the rat, the fat-induced increase in SAP activity is due predominantly to intestinal alkaline phosphatase (Saini & Posen, 1969). This paper demonstrates, also by an immunological technique, t...

Leucocyte alkaline phosphatase activity has been determined in a normal group, a group of pregnant patients, and a group of patients with ectopic pregnancy.A great rise in alkaline phosphatase occurred in pregnant patients in their third trimester, but the results were only slightly raised in the ectopic group. Five of six consecutive cases gave results which fell within the normal range. The m...

In an attempt to demonstrate a rapid and economical approach to deriving reference intervals, we analyzed data on plasma calcium and phosphate from a multichannel analyzer for more than 20 000 subjects and data on alkaline phosphatase from more than 10 000 subjects. Subjects were selected by the criterion, that their results for constituents other than the one of interest were within current re...

An organ culture method for the maintenance of rectal biopsies over a period of 24 hours is described. Good preservation of histological architecture and continued crypt cell proliferation were shown over the culture period. The colonic enzyme alkaline phosphatase was found to rise over the period of culture. This rise was dependent upon continued protein synthesis by the cell. Changes in alkal...

1. Liver and bone alkaline phosphatase isoenzymes were solubilized with the zwitterionic detergent sulphobetaine 14, and purified to homogeneity by using a monoclonal antibody previously raised against a partially-purified preparation of the liver isoenzyme. Both purified isoenzymes had a specific activity in the range 1100-1400 mumol/min per mg of protein with a subunit Mr of 80,000 determined...

Lrine specimens collected at random from normal individuals were immediately centrifuged at 2000 rpm for 5 miii., and the supernatant was then brought to a boil, thus destroying all alkaline phosphatase activity without inactivating tile inhibitor (2). The boiled urine was then divided into 2 aliquots, one of which was used undialyzed and tile other used after being dialyzed against runnilig ta...

The importance of bacterial, phytoplankton and dissolved alkaline phosphatase activity (APA) in the northern Adriatic was investigated during 2006. In surface waters total APA increased from early spring (0.07-0.08 micromol l(-1) h(-1)) to late spring (up to 4.64 micromol l(-1) h(-1)) and remained relatively high during the summer (0.46-0.71 micromol l(-1) h(-1)), due to an increase in specific...