نتایج جستجو برای: alicyclobacillus
تعداد نتایج: 237 فیلتر نتایج به سال:
Confinement of an enzyme’s active site is critical to the efficiency chemical reactions and has been recognized as important tool for catalysis accelerate by proximity via specific interactions with substrate control course reaction. Despite these benefits, a strongly confined inherently limited compounds that resemble native substrate, only small deviations tolerated. To unlock potential modif...
The aim of this work was to investigate the behavior of thermophilic esterase EST2 from Alicyclobacillus acidocaldarius in milk and cheese models. The pure enzyme was used to compare the EST2 hydrolytic activity to the activity of endogenous esterase EstA from Lactococcus lactis. The results indicate that EST2 exhibits 30-fold-higher esterase activity than EstA. As EstA has thioesterase activit...
A combination of immunomagnetic separation (IMS) and polymerase chain reaction (PCR) was used to detect Alicyclobacillus acidoterrestris (A. acidoterrestris) in apple juice. The optimum technological parameters of the IMS system were investigated. The results indicated that the immunocapture reactions could be finished in 60 min and the quantity of IMPs used for IMS was 2.5 mg/mL. Then the comb...
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