Despite the success of alginate scaffolds and mesenchymal stem cells (MSCs) therapy in cardiac failure treatment, the impact of the physicochemical environment provided by alginate matrices on cell behavior has never been investigated. The purpose of this work was double: to determine the alginate composition influence on (1) encapsulated rat MSC viability, paracrine activity, and phenotype in...

18 Alginate lyases are enzymes that degrade alginate through β-elimination of the glycosidic bond 19 into smaller oligomers. We investigated the alginate lyases from Vibrio splendidus 12B01, a 20 marine bacterioplankton species that can grow on alginate as its sole carbon source. We 21 identified, purified, and characterized four polysaccharide lyase family 7 alginates lyases – 22 AlyA, AlyB, A...

The present study assesses effects of acutely infarcted myocardium on apparent microsphere loss as a function of time, determines effects of apparent microsphere loss on blood flow measurements to ischemic regions, and determines to what extent apparent microsphere loss alters interpretation of serial measurements of collateral blood flow. Studies were performed in 35 awake mongrel dogs chronic...

the protease enzyme breaks down protein into amino acids as its constructors. protease immobilization onto appropriate support materials plays an essential role in various fields of technology including the food and detergent industries. accordingly, improvement of protease immobilization has been highly regarded due to its applications in bio catalysis. alginate and chitosan are natural polysa...

Alginate, a non-toxic polysaccharide isolated from brown algae, is a widely used 3-dimensional (3D) porous scaffold for the granulosa cell and follicle encapsulation. However, impurities in commercial alginate can lead to alginate biocompatibility reduction. The aim of this study was to evaluate in vitro behavior of the granulosa cells seeded on the purified alginate in varying concent...

Mucoid strains of Pseudomonas aeruginosa produce a high-molecular-weight exopolysaccharide called alginate that is modified by the addition of O-acetyl groups. To better understand the acetylation process, a gene involved in alginate acetylation called algF was identified in this study. We hypothesized that a gene involved in alginate acetylation would be located within the alginate biosyntheti...

We have locked a whispering-gallery resonance of a fused-silica microsphere to a frequency-scanning laser. The resonance frequency is modulated by axial compression of the microsphere, and phase-sensitive detection of the fiber-coupled optical throughput is used for locking. Using a laser wavelength of either 1570 nm or 830 nm, we demonstrate a locked tracking range exceeding 30 GHz for a micro...

This article introduced the work of ethylcellulose based polymeric microsphere loaded with nifedipine for reduction in frequency of administration with low solubility in aqueous medium and high rate of absorption in the stomach. The non-aqueous polymeric suspension was put dropwise into an aqueous medium containing polyvinyl alcohol as a surfactant for the synthesis of microsphere by solvent ev...

The purpose of this study is to examine the correlation of measured regional cerebral blood flow (rCBF) by means of a new microsphere method (non-invasive microsphere method), to the autoradiography (ARG) method, which is an established quantification method for 123I-IMP brain SPECT. The non-invasive microsphere (NIMS) method and ARG method were simultaneously applied to 30 patients, and quanti...

Quality-factors of waveguide coupled microsphere resonators of 30 μm diameter are measured. A Teflon buffer layer is deposited on the waveguide to separate the microsphere from the waveguide and the effect of this separation on the measured quality-factor is discussed. The whispering-gallery mode propagation along the cavity of the microsphere as well as the optical path lengths of the modes ar...