Sphingomonas sp. A1 directly incorporates alginate polysaccharides through a 'superchannel' comprising a pit on the cell surface, alginate-binding proteins in the periplasm and an ABC transporter (alginate importer) in the inner membrane. Alginate importer, consisting of four subunits, AlgM1, AlgM2 and two molecules of AlgS, was crystallized in the presence of the binding protein AlgQ2. Prelimi...

In this study, we developed lysosome-alginate beads for application as an oral drug delivery system (ODDS). The beads harboring lysosomes, which have antimicrobial activity, and various concentrations of alginate were characterized and optimized. For application as an ODDS, pH-dependent lysosome-alginate beads were generated, and the level of lysosome release was investigated by using antimicro...

We report on a capillary-based microfluidic platform for the fabrication of non-spherical sodium alginate microgels. The sodium alginate droplets were crosslinked off-chip in a mixture of barium acetate and glycerol solution. Novel morphologies such as tear drop, lamp-like, mushroom-like, double-dimpled and bowl-like microgels were fabricated by controlling the size, impact velocity (at the cro...

the present work investigated the preparation of biodegradable beads with alginate polymer by ionotropic gelation method to improve the control release properties of the antibiotic rifampicin. ionotropic gelation method was applied to prepare beads using calcium chloride (cacl2) as cationic component and alginate as an anionic component. in this method, adding 0.5% w/v polyvinyl alcohol (pva) t...

AIM This study investigated the effectiveness of disinfecting solution when incorporated into alginate powder instead of water against some microorganisms and on compressive strength of alginate. MATERIALS AND METHODS For measuring antimicrobial activity of alginate, 60 alginate specimens were prepared and divided into two groups: One with water incorporated in the mix (control) and the other...

BACKGROUND Adverse cardiac remodeling and progression of heart failure after myocardial infarction are associated with excessive and continuous damage to the extracellular matrix. We hypothesized that injection of in situ-forming alginate hydrogel into recent and old infarcts would provide a temporary scaffold and attenuate adverse cardiac remodeling and dysfunction. METHODS AND RESULTS We de...

Bisphosphonates are established as supportive therapy to reduce the frequency and severity of cancer-related skeletal complications. Oral formulations are preferred over intravenous if the patients are not hospitalized. The maximum absorption of oral bisphosphonates takes place in stomach. The adverse events with oral dosing are seen in buccal mucosa and gastrointestinal tract, which lead to po...

Alginate beads containing diltiazem hydrochloride (DTZ) were prepared by the ionotropic gelation method. The effects of various factors (alginate concentration, additives type, calcium chloride concentration and curing time) on the efficiency of drug loading were investigated. The formulation containing a mixture of 0.8% methylcellulose (MC) and 4% alginate cured in 2% calcium chloride for 6 h ...

The collagenous dura mater requires a secure closure following implantation of neural prosthetic devices to avoid complications due to cerebrospinal fluid leakage and infections. Alginate was previously suggested for use as a dural sealant. The liquid application and controllable gelling conditions enable alginate to conform to the unique geometries of a neural prosthetic device and the surroun...

BACKGROUND Alginate is a natural polysaccharide obtained from brown algae and has been shown to have numerous applications in biomedical science, such as wound healing, delivery of bioactive agents, and cell transplantation. Ovalbumin (OVA) peptide 323-339 has been reported to be involved in immune response.  MATERIAL AND METHODS This work investigated the use of alginate particles as a carrier...