The distribution of xanthine dehydrogenase throughout the soybean plant as well as the intercellular localization of xanthine dehydrogenase within soybean nodules was determined. Polyclonal antibodies against purified xanthine dehydrogenase were prepared and used in an enzymelinked immunosorbent assay to determine whether xanthine dehydrogenase is a nodule-specific protein. This immunological a...

Salinity, alkalinity and drought stress are the major abiotic stresses hindering the productivity of alfalfa (Medicago Sativa) in arid and semi-arid regions. Therefore an ecological survey was conducted to characterize 600 Sinirhizobium strains of diverse geographical origin, isolated from the root nodules of alfalfa (Medicago Sativa). Rhizobial isolates were studied under free living condition...

(Z ,Z)-3,9-cw -6,7-Epoxyheptadecadiene, and (Z,Z)-6,9-cw-3,4-epoxyheptadecadiene were identified by gas chrom atography/flam e ionization detec tion -e lec troan tennograph ic detec­ tion (G C /F ID -E A D ) and coupled gas ch ro m ato g rap h y -m ass spectrom etry (G C-M S) from abdom inal tip extracts o f Tephrina arenacearia (Lepidoptera: G eom etridae), an alfalfa pest. In gas chrom atogra...

Seven legume extracts containing phytoestrogens were analyzed for estrogenic activity. Methanol extracts were prepared from soybean (Glycine max L.), green bean (Phaseolus vulgaris L.), alfalfa sprout (Medicago sativa L.), mung bean sprout (Vigna radiata L.), kudzu root (Pueraria lobata L.), and red clover blossom and red clover sprout (Trifolium pratense L.). Extracts of kudzu root and red clo...

l-Phenylalanine ammonia-lyase has been purified from elicitor-treated alfalfa (Medicago sativa L.) cell suspension cultures using two protocols based on different sequences of chromatofocusing and hydrophobic interaction chromatography. Three distinct forms of the intact enzyme were separated on the basis of affinity for Octyl-Sepharose, with isoelectric points in the range pH 5.1 to 5.4. The n...

Beginning in 2000, snap bean (Phaseolus vulgaris L.) crops exhibiting virus-like symptoms on the foliage (leaf distortion, yellow and green mottling, and mosaic) were increasing in frequency on a regional scale across the northern United States and Canada (10,20). These crops also produced fewer pods or pods that were either twisted or necrotic (hence unmarketable). Across the Midwest and north...

Effects of plant-bound condensed tannin (CT)-containing sainfoin vs. CT-free alfalfa (or low-CT alfalfa-sainfoin mixture), plant stage of maturity, and their interaction on enteric methane (CH4) emissions, diet digestibility, and N excretion were studied, using 8 ruminally cannulated beef heifers in 2 sequential short-term experiments (Exp. 1 and 2). In Exp. 1, first growth legumes were harvest...

Sufficient codominant genetic markers are needed for various genetic investigations in alfalfa since the species is an outcrossing autotetraploid. With the newly developed next generation sequencing technology, a large amount of transcribed sequences of alfalfa have been generated and are available for identifying SSR markers by data mining. A total of 54,278 alfalfa non-redundant unigenes were...

Cytokinin is required for the initiation of leguminous nitrogen fixation nodules elicited by rhizobia and the delay of the leaf senescence induced by drought stress. A few free-living rhizobia have been found to produce cytokinin. However, the effects of engineered rhizobia capable of synthesizing cytokinin on host tolerance to abiotic stresses have not yet been described. In this study, two en...

A large set of genome-wide markers and a high-throughput genotyping platform can facilitate the genetic dissection of complex traits and accelerate molecular breeding applications. Previously, we identified about 0.9 million SNP markers by sequencing transcriptomes of 27 diverse alfalfa genotypes. From this SNP set, we developed an Illumina Infinium array containing 9,277 SNPs. Using this array...