Adrenocorticotropic Hormone Stimulation During Adrenal Vein Sampling To the Editor: We read with interest the article by Seccia et al,1 which investigated the effect of adrenocorticotropic hormone stimulation during adrenal vein sampling on the identification of lateralized aldosterone excess in primary aldosteronism. The authors concluded that a high or an intermediate dose of adrenocorticotro...

There is increasing evidence for rapid steroid action on electrolyte transport in human mononuclear leukocytes (HML). In HML, aldosterone stimulates the Na+/H+ antiporter within a few minutes. Because a variety of hormones and growth factors activate the Na+/H+ antiporter via protein kinase C and inositol phospholipids, a possible involvement of inositol-1,4,5-trisphosphate (IP3) in the rapid e...

Potassium is known to enhance the aldosterone-stimulating action of angiotensin II. Such a synergistic interaction of potassium with angiotensin II could represent an action by angiotensin II to potentiate potassium as a stimulus. To examine for this effect of angiotensin II on potassium, plasma aldosterone levels were measured before and after an infusion of potassium chloride (15 meq i.v.) in...

In an earlier report (1), it was suggested from cross circulation experiments that a humoral agent provides the immediate stimulus for aldosterone production. It was proposed that this humoral substance be called the aldosterone-stimulating hormone (ASH). The present experiments were designed to determine the locus of secretion of ASH. Specific regions or organs which might be the source of ASH...

Previous in vitro studies demonstrated that aldosterone rapidly activates sodium-hydrogen exchangers 1 and 3 (NHE 1 and 3). In vitro investigations revealed that protein kinase C (PKC) regulates NHE properties. We previously demonstrated that aldosterone rapidly enhances PKCα protein abundance in the rat kidney. There are no reports of renal PKCβ (I and II) protein levels related to the regulat...

Excessive production of aldosterone leads to the development of hypertension and cardiovascular disease by generating an inflammatory state that can be promoted by T cell immunity. Because nature and intensity of T cell responses is controlled by dendritic cells (DCs), it is important to evaluate whether the function of these cells can be modulated by aldosterone. In this study we show that ald...

• At the Laurentian Hormone Conference in 1958, evidence was presented to show that the immediate stimulus to aldosterone production is humoral. Cross-circulation of blood from dogs with experimental secondary hyperaldosteronism through normal isolated adrenals produced an increase in aldosterone secretion by the isolated adrenals.' This finding suggests that a humoral agent in peripheral blood...

The steady state effects of different infusion rates of aldosterone on plasma concentrations of sodium and potassium, plasma renin activity, sodium space, and mean arterial pressure were determined. Measured amounts of aldosterone were infused continuously into adrenalectomized dogs for 13 weeks. Four rates of aldosterone administration were used: 16 +/- 1 mug/day, 48 +/- 3 mug/day (approximate...

BACKGROUND Experimentally, aldosterone in association with NaCl induces cardiac fibrosis, oxidative stress, and inflammation through mineralocorticoid receptor activation; however, the biological processes regulated by aldosterone alone in the heart remain to be identified. METHODS AND RESULTS Mice were treated for 7 days with aldosterone, and then cardiac transcriptome was analyzed. Aldoster...

In an earlier study, 51% of total renal nuclear content of 3H-aldosterone was recovered bound to nuclear proteins readily extractable with Tris-3 fll~ CaC12. Modifications iu the methods used to prepare renal nuclear fractions resulted in the isolation of 3H-aldosterone bound to chromatin to the extent of 55% of the total nuclear content of the steroid. The chromatin binding system was stereosp...