In 0.05 M potassium phosphate buffer (pH 7.0, ZO”), native rabbit muscle aldolase has been found to exhibit a molecular weight (mn N g, pZ) close to 160,000, in agreement with previous findings. Upon exposure to cold alkaline borate buffer (pH 12.5, p = 0.17, 0”), the enzyme spontaneously dissociates into its monomers which subsequently undergo slow hydrolytic degradation. Compensating for elec...

The major portion of the ribose and deoxyribose of the RNA and DNA of Escherichia coli does not arise from the oxidative pathway leading from hexose to pentose.1l3 At least 70 per cent of the pentose arises from nonoxidative pathways in which glucose is converted to triose by the Embden-Meyerhof pathway.4 Triose, in the form of glyceraldehyde 3-phosphate, then receives a two-carbon unit from a ...

Aldolase B is an enzyme of the glycolytic pathway whose activity and mRNA levels in the liver fluctuate according to dietary status. Both the enzyme activity and the mRNA concentration decline during fasting and increase four- to eightfold upon refeeding of a carbohydrate-rich diet. The mechanism, however, of the mRNA induction remains unknown. To elucidate the mechanisms that regulate this ind...

Glucose 6-phosphate, fructose 6-phosphate, fructose 1, 6-diphosphate, and triose phosphates, and the enzymes phosphofructokinase, aldolase, and glucose 6-phosphate dehydrogenase were extracted from banana fruit (Musa cavendishii, Lambert var. Valery) at the (a) preclimacteric, (b) climacteric rise, (c) climacteric peak, and (d) postclimacteric stages of ripening. The level of fructose 1, 6-diph...

Protein metabolism plays an important role in plant adaptation to heat stress. This study was designed to identify heat-responsive proteins in roots associated with thermotolerance for two C3 grass species contrasting in heat tolerance, thermal Agrostis scabra and heat-sensitive Agrostis stolonifera L. Plants were exposed to 20 degrees C (control), 30 C (moderate heat stress), or 40 degrees C (...

Thiamine diphosphate (ThDP) dependent enzymes are useful catalysts for asymmetric C−C bond formation through benzoin-type condensation reactions that result in α-hydroxy ketones. A wide range of aldehydes and ketones can be used as acceptor substrates; however, the donor substrate is mostly limited to achiral α-keto acids simple aldehydes. By using a unifying retro-biosynthetic approach towards...

A new preparation of the stable barium salt of 2,5-bis(phosphonooxymethvl)-2.5-diethoxv-1.4-dioxane Ba-2 is described, which bv treatment with DOWEX 50 B+ gives dihydroxyacetone ph&phate (DHAP) in high yield. DHAP prepared by this method was used for aldolase-catalyzed condensations. An increasing number of papers dealing with enzymatic aldol reactions demonstrates their synthetic utility 1,2,4...

In 0.05 M potassium phosphate buffer (pH 7.0, ZO”), native rabbit muscle aldolase has been found to exhibit a molecular weight (mn N g, pZ) close to 160,000, in agreement with previous findings. Upon exposure to cold alkaline borate buffer (pH 12.5, p = 0.17, 0”), the enzyme spontaneously dissociates into its monomers which subsequently undergo slow hydrolytic degradation. Compensating for elec...

In 0.05 M potassium phosphate buffer (pH 7.0, ZO”), native rabbit muscle aldolase has been found to exhibit a molecular weight (mn N g, pZ) close to 160,000, in agreement with previous findings. Upon exposure to cold alkaline borate buffer (pH 12.5, p = 0.17, 0”), the enzyme spontaneously dissociates into its monomers which subsequently undergo slow hydrolytic degradation. Compensating for elec...