The influence of the synthetic phenylurea cytokinin 4-PU-30 and of the adenin cytokinin kinetin on the chilling and freezing stress of tobacco callus was studied histochemically. The investigations were carried out immediately after the low-temperature stress and after 20 days cultivation. It was established that alcohol dehydrogenase and glutamate dehydrogenase activities decreased but esteras...

Heart lipoamide dehydrogenase, liver alcohol dehydrogenase and egg-white lysozyme are photo-oxidized in the presence of various dye sensitizers. The photodynamic process is preceded by the binding between the enzyme and the sensitizers. Among the commonly used dyes, halogenated xanthines and thiazine are effective sensitizers for the photo-inactivation of these three enzymes. Histidine residues...

Twin, family, and adoption studies have consistently shown that genetic factors play an important role in the pathogenesis of alcohol dependence. Numerous studies have aimed to identify genes that contribute to susceptibility to alcohol dependence. Whole-genome linkage studies have identified several chromosomal regions that are linked with alcohol dependence. Association studies have also iden...

A bi-enzymatic cascade for the redox-isomerisation of allylic alcohol is presented. Coupling of an alcohol dehydrogenase to an enoate reductase has been successfully applied in one pot for the isomerisation of an allylic alcohol to the corresponding ketone. Critical parameters for yield and selectivity have been investigated.

PURPOSE There are many similarities in the clinical presentation of Leber hereditary optic neuropathy (LHON) and in patients who have optic neuropathy and a history of heavy tobacco and alcohol consumption. The main objective of this study is to investigate the frequency of primary and secondary mitochondrial DNA (mtDNA) mutations for LHON in patients diagnosed as having alcohol and tobacco opt...

The gene loci ehyAB, calA, and calB, encoding eugenol hydroxylase, coniferyl alcohol dehydrogenase, and coniferyl aldehyde dehydrogenase, respectively, which are involved in the first steps of eugenol catabolism in Pseudomonas sp. strain HR199, were amplified by PCR and combined to construct a catabolic gene cassette. This gene cassette was cloned in the newly designed broad-host-range vector p...

An elevated cholesterol level might lead to cardiovascular disease (CVD). Statins block the cholesterol synthesis pathway in the liver. Atorvastatin is the most widespread statin worldwide and, its chemical synthesis requires toxic catalysts, resulting in environmental pollution. Hence, enzymatic synthesis of atorvastatin is desirable. This process could be done by Lactobacillus kefir ...

mRNA steady-state levels and activities of enzymes of intermediary carbon metabolism (hexokinase, phosphoglucoisomerase, phosphofructokinase, glucose-6-phosphate dehydrogenase, phosphoglucomutase) and glucose-regulated enzymes (pyruvate decarboxylase, pyruvate dehydrogenase, invertase, alcohol dehydrogenase) were determined in glucose-limited continuous cultures of an industrial strain of Sacch...

THE GUIDELINES FOR MANAGEMENT OF ETHANOL, METHANOL AND ETHYLENE GLYCOL POISONING AT THE EMERGENCY ROOM By Niru Prasad, M.D., F.A.A.P., F.A.C.E.P. Department of Emergency Medicine Henry Ford Hospital West Bloomfield Center Department of Ambulatory Pediatrics St. Joseph Mercy Hospital Pontiac Ethanol Overdose Introduction Pharmacokinetics Absorption and Metabolism Clinical Symptoms Produced by Et...