In this study, methanol and ether extracts of five endemic Asperula species (Rubiaceae) from Turkey (A. antalyensis, A. brevifolia, A. pseudochlorantha, A. purpurea subsp. apiculata and A. serotina), used in the traditional system of medicine, were tested for antimicrobial activity by the agar well diffusion method and the broth dilution method. The most active species were Asperula brevifolia ...

This study was an attempt to develop a fluorescent-antibody (FA) test to differentiate Actinomyces israelii and A. naeslundii as an aid in their laboratory identification. Two strains of A. israelii (X522 and A601) and two strains of A. naeslundii (X454 and X600), which had received intensive study by several investigators, were used for the immunization of rabbits. Working titers, based on tes...

background and purpose: the status of medicinal plants in iranian traditional medicine and rich existence of herbal sources, on one hand, and current problems in curing infections, on the other hand, are the factors to study medicinal plants more accurately. tarragon (artemisia dracunculus l.) is a herbaceous plant from asteraceae family, available in planted and farming forms in iran. this stu...

A 25-microg fluconazole disk diffusion test using a Mueller-Hinton agar plate containing 2% glucose and 5 microg of methylene blue/ml (GM-MH) was compared to the macrodilution reference method for 210 Candida species. The GM-MH agar plate was read at 24 h. The predictive values of disks with susceptible, intermediate, and resistant results on the GM-MH agar plate at 24 h were 97.1, 56.3, and 76...

The bacterial action of gentamicin and that of a mixture of gentamicin and 15-nm colloidal-gold particles on Escherichia coli K12 was examined by the agar-well-diffusion method, enumeration of colony-forming units, and turbidimetry. Addition of gentamicin to colloidal gold changed the gold color and extinction spectrum. Within the experimental errors, there were no significant differences in an...

Susceptibility of Neisseria gonorrhoeae to gentamicin, the primary treatment for gonorrhea in Malawi since 1993, was determined by using agar dilution MICs, E-test MICs, disc diffusion, and clinical cure rate. Agar dilution MICs were slightly higher in 1996 than in 1993 isolates, with a concomitant drop in the clinical cure rate. E-test MICs were substantially lower than agar dilution determina...

The results of disk diffusion and plate dilution susceptibility testing of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Proteus mirabilis on media gelled with agar-agar or with a synthetic hydrogel were compared. Synthetic hydrogel can be combined with a totally defined synthetic amino acid medium to yield a reproducible, totally defined, synthetic solid medium without t...

objective: the present study was carried out to determine the most potent available antimicrobials in the treatment of pathogens of reproductive health importance in nigeria. material and methods: using the agar disc-diffusion and modified agar well-diffusion methods, bacterial and fungal pathogens obtained from clinical specimens of female patients presenting at the university college hospital...

  BackgroundandObjectives:RapidandaccuratedetectionofmethicillinresistantStaphylococcus aureus (MRSA) is an important role of clinical microbiology laboratories to avoid treatment failure. The aim of this study was to compare conventional methods against the E-test minimum inhibitory concentration (MIC) method to determine the best phenotypic method. Materials a...

background and objectives: antibacterial susceptibility testing of clinical bacterial isolates through disk diffusion method plays a major role in antibacterial treatment. one of the main factors affecting the result of these tests is the type, structure and quality of the disks. the main objective of this study was to compare the agreement of antibiotic disks originated from three companies on...