21 The agar dilution method has been standardized by the CLSI for the susceptibility testing of 22 Campylobacter species, and according to these standards, the disk diffusion method should only 23 be used for the screening of macrolide and ciprofloxacin resistance. Nevertheless, the disk 24 diffusion test is currently widely used, since it is easy to perform in clinical microbiology 25 laborato...

Diameter reductions accompanying preincubation, and funnel-shaped diffusion zones from agar wells, readily demonstrate chemical and physical instability of preparations in antimicrobial analyses.

The effects of agars on the determination of the potency of polymyxin B sulfate by the agar plate diffusion method were investigated. The results showed that there were significant differences in the potency of polymyxin B sulfate between agars produced by different manufacturers. In order to investigate this phenomenon, two major components of polymyxin B sulfate, namely, the polymyxin B1 and ...

Fluoroquinolone resistance appears to be increasing in many species of bacteria, particularly in those causing nosocomial infections. However, the accuracy of some antimicrobial susceptibility testing methods for detecting fluoroquinolone resistance remains uncertain. Therefore, we compared the accuracy of the results of agar dilution, disk diffusion, MicroScan Walk Away Neg Combo 15 convention...

Background and Purpose: Actinomycetes have been discovered as source of antifungal compounds that are currently in clinical use. Invasive aspergillosis (IA) due to Aspergillus fumigatus has been identified as individual drug-resistant Aspergillus spp. to be an emerging pathogen opportunities a global scale. This paper described the antifungal activity of one terrestrial actinomycete against the...

This paper introduces a reaction-diffusion-chemotaxis model for bacterial aggregation patterns on the surface of thin agar plates. It is based on the non-linear degenerate cross diffusion model proposed by Kawasaki et al. [1] and it includes a suitable nutrient chemotactic term compatible with such type of diffusion. High resolution numerical simulations using Graphic Processing Units (GPUs) of...

The rate of diffusion of a protein in solution from a source in agar gel is, within limits, proportional to its original concentration (Ouchterlony, 1949). When plasminogen in biological fluids is allowed to diffuse from a source in agar/fibrinogen gel for a given time, the distance diffused can be ascertained by activation of the plasminogen to plasmin by streptokinase after diffusion has take...

l. A diabetogenic protein isolated from bovine adenohypophysis which had previously been demonstrated to cause fasting hyperglycemia and decreased glucose tolerance in dogs and man is highly antigenic when injected into rabbits. 2. Immunoelectrophoresis and gel double diffusion studies did not provide evidence of multiple antibodies or antigens in the bovine diabetogenic protein: anti-bovine di...

We evaluated the NCCLS M44-P fluconazole disk diffusion method in comparison with the NCCLS M27-A2 broth microdilution method for determining the susceptibility of 276 isolates of Cryptococcus neoformans. Disk diffusion testing was performed using Mueller-Hinton agar supplemented with 2% glucose and 0.5 microg of methylene blue/ml. Among the 276 isolates, 259 (93.8%) were susceptible, 16 (5.8%)...