As an approach to study the mechanism of the microbody transition (glyoxysomes to leaf peroxisomes) in greening pumpkin cotyledons, catalase molecules were purified from the two different types of microbody and their structural properties were compared. The purified glyoxysomal catalase was found to consist of four identical subunits (55 kDa), whereas the leaf peroxisomal catalase contains two ...

The production of olive oil generates wastewater with a high organic load and toxicity due to the concentration phenolic compounds. In recent years, study on treatments these waters has been intensified together search for process recover compounds their great antioxidant potential. All this aim implementing concept circular economy. study, four different ultrafiltration membranes were evaluate...

Proteins from underutilized defatted oilseed meals have been proposed as promising sources of bioactive peptides. This study was conducted to determine the anti-inflammatory activities five proteins (flaxseed, rapeseed, sunflower, sesame and soybean) hydrolysed via alcalase, pepsin (at pH 1.3 2.1), respectively, compare these against two dairy (whey, casein). The potential protein hydrolysates ...

In this issue, Verdier et al comment on our recent publication, concluding that some anti-EpoR antibodies have limited utility for detecting EpoR expression. 1 We reported that the C-20 anti-EpoR antibody from Santa Cruz Biotechnology (Santa Cruz, CA) detected 5 protein bands in UT-7/Epo cells including 59-kDa and 66-kDa proteins. Using both direct and indirect methods, we showed that the 59-kD...

A procedure was developed for the immunoprecipitation of glycosylated and nonglycosylated forms of the insulin receptor and its precursors without prior purification using lectins. 3T3-L1 adipocytes were labeled with [35S]methionine after which 35S-labeled receptor polypeptides were specifically immunoprecipitated and characterized by sodium dodecyl sulfatepolyacrylamide gel electrophoresis. Th...

snail is one of the worst causes of food allergy. thus, the aim of this study was to identify the major and minor allergens of the local marine snail ( cerithidea obtusa ) and subsequently to investigate the impacts of heat treatment on the ige-binding activity of snail allergens. proteins from raw and heat-treated snails (boiled, roasted and fried) were extracted and then resolved by sodium do...

Alterations in the genomic position of the tobacco mosaic virus (TMV) genes encoding the 30-kDa cell-to-cell movement protein or the coat protein greatly affected their expression. Higher production of 30-kDa protein was correlated with increased proximity of the gene to the viral 3' terminus. A mutant placing the 30-kDa open reading frame 207 nucleotides nearer the 3' terminus produced at leas...

The adenovirus E1B 55-kDa and E4 34-kDa oncoproteins bind and inactivate the p53 tumor suppressor gene product, resulting in cell transformation. A recently discovered cellular protein, p73, shows extensive similarities to p53 in structure and function. Here we show that the simultaneous transient expression of E1B 55-kDa and E4 34-kDa proteins is sufficient to drastically shorten the intracell...

This study aimed to compare adherence dietary guidelines between elderly and non-elderly individuals with type 2 diabetes mellitus (T2DM) in Korea. Data of 4,577 participants T2DM were collected from the 1998-2015 Korea National Health Nutrition Examination Surveys. The classified into two groups based on age: group comprising aged 30-64 years ≥ 65 years. Adherence was assessed using Korean Dia...