Synthetic oligonucleotide primers were used in a polymerase chain reaction (PCR) technique to detect the gene for aerolysin in strains of Aeromonas hydrophila and to screen for identical genes in A. caviae, A. sobria, and A. veronii isolated from patients with diarrheal disease. Primers targeted a 209-bp fragment of the aer gene coding for the beta-hemolysin and detected template DNA only in th...

Pseudomonas aeruginosa ExoU, a type III secretory toxin and major virulence factor with patatin-like phospholipase activity, is responsible for acute lung injury and sepsis in immunocompromised patients. Through use of a recently updated bacterial genome database, protein sequences predicted to be homologous to Ps. aeruginosa ExoU were identified in 17 other Pseudomonas species (Ps. fluorescens...

a bacterial septicaemia occurred in 3-female oscars (astronotus ocellatus) of about 2-year old held in 300l freshwater aquaria in south tehran. the external signs of infected fish consisted of lethargy, anaemia, distension (clinical dropsy), exophthalmia and cataract. internally there was a large amount of red-ascitic fluid accumulated in the abdominal cavity with haemorrhages/ hyperaemia of in...

The aim of the study was to determine the relationship between serogroups, species, and virulence of Aeromonas sp. Isolates from common carp and rainbow trout were tested for species designation and virulence phenotype and then serogrouped. A total of 558 isolates were tested. The bacteria were identified to species level using PCR-RFLP method. The β-haemolysin, gelatinase, and caseinase activi...

Aloe vera variety barbadensis is a potential alternative antimicrobial plant extract that has been widely used in humans. The objective of this study was to investigate the susceptibility selected bacterial isolates from cultured freshwater fish different concentrations crude extract. Using Minimum inhibitory concentration method by Broth dilution technique, diluted distilled water 250 mg/ml, 1...

Aeromonas , a ubiquitous taxon in water environments, is emerging as foodborne pathogen of concern that remains understudied and under-reported. We evaluated the distribution 331 spp. isolates collected from irrigation over one year characterised their virulence profile, attachment ability to persist on lettuce. Water sources included non-tidal tidal river, farm pond reclaimed water. Twenty spe...

A total of 74 phenotypically identified presumptive motile Aeromonas isolates recovered from septicaemic freshwater ornamental fish in Sri Lanka were genetically characterized by sequencing of rpoD and gyrB genes. rpoD/gyrB phylogeny confirmed only 53 isolates as Aeromonas, among which A. veronii was the predominant species (79.2%), followed by A. hydrophila (7.5%), A. caviae (5.7%), A. jandaei...