A total of 103 strains of Aeromonas spp. isolated from clinical and from environmental samples was compared by using SDS-PAGE of periplasmic proteins patterns. Strains isolated from Polish children suffering from gastroenteritis did not appear similar to strains isolated from human living in Hong-Kong. Aeromonas sp. strains did not show a tendency to cluster according to their origin. Our resul...

In a period of one year, in a general hospital, Aeromonas hydrophila was isolated from 13 patients and Aeromonas shigelloides from one patient. Eight of the patients had superficial infections, two had urinary tract infections, and four had bacteriaemia. The association of Aeromonas bacteriaemia with cirrhosis of the liver and malignant disease, which has been previously reported, was observed ...

Human diseases caused by species of Aeromonas have been classified into two major groups: septicemia and gastroenteritis. In this study, we reported the molecular and functional characterization of a new virulence factor, ToxR-regulated lipoprotein, or TagA, from a diarrheal isolate, SSU, of Aeromonas hydrophila. The tagA gene of A. hydrophila exhibited 60% identity with that of a recently iden...

Cytolytic toxins produced by Aeromonas hydrophila and Aeromonas veronii biotype sobria strains were partially purified from culture filtrates by two steps of purification: ammonium sulfate precipitation and hydrophobic chromatography using Phenyl-Sepharose CL-4B. Hemolytic activity was detected in one or two peaks in elution profile. Purified toxins were also cytotoxic to Vero and CHO cells. Mo...

Two separate species of Aeromonas, A. sobria (not listed as a species in Bergey's Manual of Determinative Bacteriology, 8th ed.) and A. hydrophila, were primary pathogens isolated from the leg wound of a diver conducting operations in polluted waters. This is the first recorded instance of a primary infection of soft tissue in a human caused by two species of Aeromonas, one of which was resista...

The manner in which leeches are maintained before they are used for therapy has not been studied as a factor contributing to nosocomial infections. A 5-year retrospective survey of Aeromonas hydrophila nosocomial infections at a hospital in Marseille, France, revealed infections in 5 (4.1%) of an estimated 122 patients treated with leeches in the Hand Surgery Unit and 2 (2.4%) of an estimated 8...

Synthetic oligonucleotide primers were used in a polymerase chain reaction (PCR) technique to detect the gene for aerolysin in strains of Aeromonas hydrophila and to screen for identical genes in A. caviae, A. sobria, and A. veronii isolated from patients with diarrheal disease. Primers targeted a 209-bp fragment of the aer gene coding for the beta-hemolysin and detected template DNA only in th...

Aeromonas spp., considered as emerging opportunistic pathogens, belong to the family Vibrionaceae. Among the criteria currently used for their classification is the presence of a single FeSOD (iron-containing superoxide dismutase), which distinguishes them from Enterobacteriacea. In this paper the cloning of the sodA and sodB genes encoding two different SODs in Aeromonas hydrophila ATCC 7966 i...

Aeromonas hydrophila was obtained from various sources and identified on the basis of growth on selective media, phenotypic and biochemical characters. The isolates (Ah1-Ah9) were subjected to antibiotic susceptibility and resistance pattern tests. The isolates showing maximum resistance to most of the commercial antibiotic discs used were selected. The selected isolate (Ah7) was further subjec...