Adenosine deaminase (adenosine aminohydrolase EC 3.5.4.4) has been purified 468,000-fold from pooled human erythrocytes. The procedure developed was used to isolate the enzyme from up to 23 liters of packed erythrocytes at one time. An easily prepared affinity column bed material employing adenosine as the ligand was used as the final step in the purification. During elution from the affinity c...

It has been known for a number of years that there are two principal processes whereby Escherichia coli acts on adenosine (1,2) : deamination to inosine and cleavage to adenine and sugar. Later work (3-7) indicated that the cleavage was phosphorolytic in nature, as had been shown by Kalckar (8) to be the case with a mammalian enzyme. In E. coli there appears to be a number of phosphorylases act...

The mechanism of the depletion of ATP, recorded in the erythrocytes of adenosine deaminase-deficient children and of leukemia patients treated with deoxycoformycin, was investigated in normal human erythrocytes treated with this inhibitor of adenosine deaminase. Deoxyadenosine, which accumulates in both clinical conditions, provoked a dose-dependent accumulation of dATP, depletion of ATP, and i...

The mechanism of the depletion of ATP, recorded in the erythrocytes of adenosine deaminase-deficient children and of leukemia patients treated with deoxycoformycin, »asinvestigated in normal human eryth rocytes treated with this inhibitor of adenosine deaminase. Deoxyadeno sine, which accumulates in both clinical conditions, provoked a dosedependent accumulation of dATP, depletion of ATP, and ...

The primary goal of this study was to determine whether the slowing of atrioventricular (AV) conduction by ATP is caused by ATP per se or is mediated by adenosine formed from ATP degradation. We assessed the effects of ATP, beta, gamma-methylene ATP, ADP, AMP, and adenosine on AV conduction time in the isolated perfused guinea pig heart. The cardiac effluent was collected and analyzed for its c...

The isoenzymes ADA1 and ADA2 of the enzyme adenosine deaminase (ADA 3.5.4.4) deaminate mainly two nucleosides: adenosine and 2'deoxyadenosine, producing inosine and 2'deoxyinosine. Adenosine and 2'deoxyadenosine are molecules with many effects on human cells [1–4]. Thus, the homeostasis of these substances and the activity of the isoenzymes ADA1 and ADA2 in human cells are of extreme importance.

BACKGROUND Acid phosphatase locus 1 and adenosine deaminase locus 1 polymorphisms show cooperative effects on glucose metabolism and immunological functions. The recent observation of cooperation between the two systems on susceptibility to repeated spontaneous miscarriage prompted us to search for possible interactional effects between these genes and the correlation between birth weight and p...

In the course of studying the phosphoric acid esterases from calf intestinal mucosa (1, 2) the preparations were found to contain a potent deaminase for adenosine. The method of preparation is simple and the procedure indicates that this enzyme is quite stable. This deaminase is probably identical with that recently prepared by Brady (3) from the same material by a somewhat different procedure....

We studied the activity of adenosine deaminase in the peritoneal fluid of 66 patients who were divided into five groups according to causes of ascites as follows: tuberculous peritonitis (group I), septic peritonitis (group II), secondary to malignant tumours (group III), miscellaneous conditions (group IV), and control subjects of transudates (group V). In patients with tuberculous peritonitis...