objective: micrornas (mirnas) are single-stranded small rnas 18-25 nucleotides in length that regulate gene expression through translational inhibition and mrna cleavage. aberrant expression of mirnas contribute to several diseases. this has increased interest in profiling the expressions of these molecules. real-time quantitative pcr (rq-pcr) is a sensitive, quantitative technique for gene exp...

the objective of this study was to investigate the genotypic characteristics of campylobacter isolates in shiraz, iran. a total of 40 campylobacter isolates including 20 c. jejuni and 20 c. coli were recovered from both cattle and sheep faeces by cultivation methods. the isolates were identified on the basis of polymerase chain reaction (pcr) detection of 16srrna and multiplex pcr to determine ...

background: several virulence factors have been described in pathogenesis of staphylococcus aureus strains. staphylococcal enterotoxins(ses) that is one of the most factors, are belonging to important members of bacterial superantigens. superantigens are defined by their ability to stimulate cytokine release from both t cells and macrophages. 13 different ses are known that their function is no...

colibacillosis caused by e.coli is one of the most common illnesses in commercial chickens and induce high financial losses in the poultry industry due to morbidity, mortality and antibiotic expenses because of overuses. e.coli can mostly cause disease as a secondary or opportunistic pathogen, while certain strains with specific virulence factors even as primary pathogen can cause colibacillosi...

the aim of this study was to evaluate the ability of 15 serotypes of salmonella to form biofilm on polystyrene, polyvinyl chloride (pvc) and glass surfaces. .initially slime production was assessed on cra agar and hydrophobicity of 20 salmonella strains isolated from poultry and human and two salmonella enterica serovar typhimurium references strains was achieved by microbial adhesion to n-hexa...

objective(s): stem cell differentiation into different cell lineages depends upon several factors, cell cycle control elements and intracellular signaling elements, including p15ink4b and p16ink4a genes. epigenetics may be regarded as a control mechanism which is affected by these factors with respect to their promoter structure.   materials and methods: the cd34 + cord blood stem cells were pu...

conclusions the distribution patterns of phase-variable genes in pakistani h. pylori strains were found to be somewhat different. the dominant prevalence of the hsdr gene was an interesting finding, considering its role in bacterial defense in both micro- and macroenvironments. results on culturing, the prevalence of h. pylori infections in the samples was 44.7%. the prevalence was higher in fe...

ADAM10 is a disintegrin metalloproteinase that processes amyloid precursor protein and ErbB ligands and is involved in the shedding of many type I and type II single membrane-spanning proteins. Like tumor necrosis factor-alpha-converting enzyme (TACE or ADAM17), ADAM10 is expressed as a zymogen, and removal of the prodomain results in its activation. Here we report that the recombinant mouse AD...

ADAMs (a disintegrin and metalloproteinases) are involved in various biological events such as cell adhesion, migration and invasion, membrane protein shedding and proteolysis. However, there have been no systematic studies on the expression of ADAMs in human ovarian carcinomas. We therefore examined mRNA expression of all the proteolytic ADAM species including ADAM8, 9, 10, 12, 15, 17, 19, 20,...