The relationship between adenosine deaminase (ADA) and a human membrane thymus leukemia (HTL) antigen-detected by an antithymocyte serum was explored. A freeze-thaw extract of the T-cell-derived cell line, MOLT 4, was applied to an immunoabsorbant column of a rabbit antiserum to calf ADA. The bound MOLT 4 ADA was eluted with 6 M urea. The recovered ADA had a specific activity of 490 mumol of ad...

Intracellular adenosine deaminase (ADA), purine nucleoside phosphorylase (PNP) and terminal deoxynucleotidyl transferase (TdT) activities were investigated in adult patients with acute myelogenous leukemia (AML) in order to relate these enzymatic activities to the stage of differentiation and maturation and to the clinical outcome of AML. Both ADA and PNP were measured spectrophotometrically us...

The C-terminal domain of the Escherichia coli Ada protein (Ada-C) aids in the maintenance of genomic integrity by efficiently repairing pre-mutagenic O:(6)-alkylguanine lesions in DNA. Structural and thermodynamic studies were carried out to obtain a model of the DNA-binding process. Nuclear magnetic resonance (NMR) studies map the DNA-binding site to helix 5, and a loop region (residues 151-16...

This article is based on an Ada framework for multi-moded real-time applications called GIA (Giotto in Ada). As the name indicates it is based on the Giotto programming model, developed at the University of Berkeley. Within the GIA project original Giotto has been hosted in Ada, strictly speaking SPARK a safe subset of Ada, and has subsequently been extended. One absence of Giotto is its protoc...

Prokrustes hat allerdings, schlau wie er ist (er stammt ja schließlich aus einer uralten Räuberfamilie), inzwischen den Wechselapparat gegen den eines anderen Herstellers ausgetauscht. Als Alexeus nachzählt, traut er seinen Augen nicht: Prokrustes hat unseren armen Handlungsreisenden um die Hälfte seiner Bits betrogen (gemäß der Devise: Wer wird denn eine Bank überfallen, wenn das Gründen und B...

Thymocyte development past the CD4(-)CD8(-) stage is markedly inhibited in adenosine deaminase-deficient (ADA-deficient) murine fetal thymic organ cultures (FTOCs) due to the accumulation of ADA substrates derived from thymocytes failing developmental checkpoints. Such cultures can be rescued by overexpression of Bcl-2, suggesting that apoptosis is an important component of the mechanism by whi...

This article compares the conventional model predictive control (MPC) and active disturbance rejection (ADRC) with a novel MPADRC technique for controlling non-minimum phase behavior in DC–DC boost converter. The of converter is challenging as it nonlinear, shows continuous conduction mode (CCM). Moreover, this article, comparison presented two-phase interleaved using MPC ADRC, effectiveness in...

Due to the relatively poor cell-material interaction of alginate hydrogel, alginate-gelatin crosslinked (ADA-GEL) hydrogel was synthesized through covalent crosslinking of alginate di-aldehyde (ADA) with gelatin that supported cell attachment, spreading and proliferation. This study highlights the evaluation of the physico-chemical properties of synthesized ADA-GEL hydrogels of different compos...

Adenosine, a signaling nucleoside, exhibits tissue-protective and tissue-destructive effects. Adenosine levels in tissues are controlled in part by the enzyme adenosine deaminase (ADA). ADA-deficient mice accumulate adenosine levels in multiple tissues, including the lung, where adenosine contributes to the development of pulmonary inflammation and chronic airway remodeling. The present study d...

Adenosine deaminase (ADA) activity and tuberculostearic acid (TSA) levels in pleural effusions were measured in 18 patients with active tuberculous pleuritis, 16 patients suspected of having tuberculous pleuritis, 14 patients with carcinomatous pleuritis, and 19 patients suffering from pleuritis of non-malignant and non-tuberculous etiology. In the patients with active tuberculous pleuritis, AD...