Human peripheral blood lymphocytes are stained with the metachromatic dye acridine orange and the fluorescence of individual cells is measured by flow cytometry. The relative content of stainable RNA per cell is estimated by comparison with RNase-treated cells. Non-T and T lymphocytes have different mean quantities of RNA per cell, and these classes exhibit different distributions of RNA conten...

We report data demonstrating that zebrafish embryos irradiated by alpha particles can release a stress signal into the water, which can be communicated to the unirradiated zebrafish embryos sharing the same water medium and thereby inducing a radioadaptive response in these unirradiated zebrafish embryos. The effects of radiation on the whole embryos were studied through quantification of apopt...

In the title complex, [PdCl(2)(C(13)H(9)N)(2)], the Pd(II) ion is four-coordinated in an essentially square-planar environment by two N atoms from two acridine ligands and two Cl(-) anions. The Pd atom is located on an inversion centre, and thus the asymmetric unit contains one half of the complex and the PdN(2)Cl(2) unit is exactly planar. The dihedral angle between the PdN(2)Cl(2) unit and th...

In the mol-ecule of the title compound, C(21)H(19)NO, the terminal saturated six-membered ring of the dihydro-acridine unit adopts an envelope conformation, while the other two fused rings are nearly coplanar, with a dihedral angle of 2.61 (3)°. The coplanar ring system is oriented with respect to the phenyl ring at a dihedral angle of 74.58 (3)°. In the crystal structure, there is a C-H⋯π cont...

In the title complex, [PtI(2)(C(13)H(9)N)(C(2)H(6)OS)], the Pt(II) atom is four-coordinated in an essentially square-planar environment defined by the N atom of the acridine ligand, the S atom of dimethyl sulfoxide, and two iodide ions. The dihedral angle between the nearly planar PtI(2)NS unit [maximum deviation = 0.083 (2) Å] and the acridine ligand [maximum deviation = 0.038 (6) Å] is 89.29 ...

In the crystal structure of the title compound, C(19)H(14)N(+)·CF(3)SO(3) (-), the cations are linked to each other by very weak C-H⋯π inter-actions, while the cations and anions are connected by N-H⋯O, C-H⋯O and S-O⋯π inter-actions. The acridine ring system and the phenyl ring are oriented at an angle of 80.1 (1)° with respect to each other. The mean planes of adjacent acridine units are eithe...

Lanyi, Janos K. (Stanford University School of Medicine, Palo Alto, Calif.), and Joshua Lederberg. Fluorescent method for the detection of excreted ribonuclease around bacterial colonies. J. Bacteriol. 92:1469-1472. 1966.-A test for the release of extracellular ribonuclease by Bacillus subtilis colonles was developed. The method consists of incorporating acridine orange and ribonucleic acid int...

Acridine orange (AO) has been used to vitally stain somatic and germinal nuclei in vivo (1, 2) and to differentially fluorochrome living and dead bacteria, yeast, and onion root tip cells. The latter phenomenon was discovered by Strugger, whose book, Fluoreszenzmikroskopie und Mikrobiologie (7), details the technics required for adapting the metafluorochromatic staining as a test of cell viabil...

To determine whether acridine orange (AO) staining of blood cultures could be used as a substitute for blind subculture when used in conjunction with the BACTEC system (Johnston Laboratories, Inc., Towson, Md.), the two methods were compared on all BACTEC-negative specimens. Since blind subcultures were routinely performed in our laboratory on days 2 and 6 of incubation, AO staining was also pe...