Abstract Sanfilippo B is a lysosomal disorder characterized by the pathological accumulation of heparan sulfate. It caused mutations in NAGLU gene that codes for alpha-N-acetylglucosaminidase enzyme. The objective this study was to determine reference values and frequency Colombia through an enzyme analysis leukocytes extracts. We aim inform community health system so they can work preventive w...

This work reports the characterization of the first known peptidoglycan hydrolase (Acp) produced mainly during vegetative growth of Clostridium perfringens. Acp has a modular structure with three domains: a signal peptide domain, an N-terminal domain with repeated sequences, and a C-terminal catalytic domain. The purified recombinant catalytic domain of Acp displayed lytic activity on the cell ...

Rotavirus, a non-enveloped reovirus, buds into the rough endoplasmic reticulum and transiently acquires a membrane. The structural glycoprotein, VP7, a 38-kD integral membrane protein of the endoplasmic reticulum (ER), presumably transfers to virus in this process. The gene for VP7 potentially encodes a protein of 326 amino acids which has two tandem hydrophobic domains at the NH2-terminal, eac...

Following the original observation of Beaufay et al. 3, the presence of lysosomes in brain has been amply documented and a lysosomal localization for the following acid hydrolases establishedlS,18,Zl,27,28: acid phosphatase (EC 3.1.3.2), fl-2-acetylamino-2-deoxy-D-glucohydrolase (N-acetylglucosaminidase) (EC 3.2.1.30), fl-galactosidase (EC 3.2.l.23), fl-glucuronidase (EC 3.2.1.31), acid ribonuc...

Nine strains of Streptococcus oralis, isolated from blood cultures of patients with infective endocarditis or from the oral cavity as part of the normal flora, were examined for their ability to elaborate sialidase (neuraminidase) and N-acetylglucosaminidase, enzymes which are involved in the degradation of glycoproteins. Both glycosidases were induced when bacteria were grown in a minimal medi...

Phosphomannosyl residues on acid hydrolases serve as recognition markers which target these enzymes to lysosomes. We have found that the oligosaccharide units of newly synthesized beta-glucuronidase contain phosphate residues in diester linkage between mannose and alpha-linked N-acetylglucosamine residues (Tabas, I., and Kornfeld, S. (1980) J. Biol. Chem. 255, 6633--6639). To obtain larger amou...