Studies are elaborated on regulation of acetylcholinesterase activity by nerve growth factor (NGF) in cultures of clonal PC12 pheochromocytoma cells. Acetylcholinesterase specific activity in these cultures is maximally and half-maximally increased by NGF concentrations of approximately 1-1.5 and 0.5 nM, respectively. These increases are blocked by antiserum to NGF and are neither mimicked nor ...

A concept of tandem driven dynamic self-inhibition is demonstrated through dynamic inhibitors of acetylcholinesterase (AChE) using reversible transthiolesterification.

When Robert Laskowski, MD, president and CEO of Christiana Care Health System, Wilmington, Del., and an ACHE Member, speaks to new employees as part of their orientation, he talks about the organization’s mission, commonly referred to as the Christiana Care Way. It is a framework, a compass if you will, that gets employees and the institution on the right path—the way—and keeps them there: “We ...

We introduce a one-step histochemical method with cobalt as the precipitating agent for ferrocyanide for the light microscopic demonstration of acetylcholinesterase activity. This method was used to demonstrate acetylcholinesterase in normal cortical fibers and neurons, as well as pathological elements such as plaques and tangles. This procedure can also be easily combined with immunohistochemi...

The synthesis, kinetic, and spectral characterization of (7-nitrobenz-2-oxa-1,3-diazole)aminoethyl and (7nitrobenz-2-oxa1,3-diazole)aminopentyl methylphosphonofluoridate are described. These homologous organophosphorous agents contain the environmentally sensitive 7-nitrobenz-2-oxa1,3-diazole chromophore. They inhibit acetylcholinesterase from Torpedo at rates exceeding lo’ M” min” to form l...