Filariasis is a major health problem, affecting millions of people in tropical and subtropical regions of the world. Targeting the parasite specific enzyme is not only rational but essential for developing effective control measure against filariasis. In our earlier studies we have identified two isozymic forms of acetylcholinesterase (AchE) from bovine filarial parasites (Setaria cervi) differ...

The synthesis, kinetic, and spectral characterization of (7-nitrobenz-2-oxa-1,3-diazole)aminoethyl and (7nitrobenz-2-oxa1,3-diazole)aminopentyl methylphosphonofluoridate are described. These homologous organophosphorous agents contain the environmentally sensitive 7-nitrobenz-2-oxa1,3-diazole chromophore. They inhibit acetylcholinesterase from Torpedo at rates exceeding lo’ M” min” to form l...

Acetylcholinesterase (AChE) inhibition is important because of its health-related implications. Three porphyrin derivatives, Tetraphenylporphinesulfonate (TPPS), 5, 10, 15, 20-Tetrakis (4sulfonatophenyl) porphyrinato Iron (III) Chloride (FeTPPS) and 5, 10, 15, 20-Tetrakis (4-sulfonatophenyl) porphyrinato Iron (III) nitrosyl Chloride (FeNOTPPS), were tested by Molecular Docking as inhibitors of ...

BACKGROUND Neurological disturbances have been implicated with aspartame (ASP) consumption and the cholinergic system with acetylcholinesterase (AChE) seems actively involved. AIM To evaluate the effect of ASP and its metabolites on rat hippocampal AChE activity. METHODS Hippocampal homogenate or pure enzyme AChE (eel E. electricus) was incubated with the sum or each of ASP components, phen...

acetylcholinesterase (ache) is a large glycoprotein that, aside from its known cholinolytic activity, co-exists with other transmitter systems and possesses other functions. in the present study, the effects of short-term rapid-eye-movement sleep deprivation (rem-sd) on ache activity in the anterior hypothalamic area have been investigated. using the flower-pot method, adult male albino rats we...

This paper presents a protocol for routine assays of human blood cholinesterase activities which separates erythrocytes from plasma by centrifugation and measures acetylcholinesterase activity in unwashed erythrocytes and butyrylcholinesterase activity in the plasma. The recommended substrate for both enzymes is 1.0 mM acetylthiocholine. The protocol is compared with other two recommended proto...

Plasma cholinesterase and erythrocyte acetylcholinesterase activities have a long history of use in monitoring both workers at risk of organophosphorus pesticide (OP) exposure and in investigating accidental exposures to OPs. On account of wide inter-individual variation, the establishment of unexposed, baseline enzyme activities is necessary for accurate interpretation. This paper describes th...

The aim of the present paper is to describe the morphology and topography of the nuclei of the amygdaloid complex in the rabbit. In the current study we also investigated the intensity of the enzymatic reaction for acetylcholinesterase (AChE) in the amygdaloid complex and the morphology of its neurones. Material consisted of 5 brains of adult New Zealand rabbit, stained either with cresyl viole...