The pyridoxal phosphate-dependent enzyme 1-aminocyclopropane-1-carboxylate synthase (ACC synthase; S-adenosyl-L-methionine methylthioadenosine-lyase, EC 4.4.1.14) catalyzes the conversion of S-adenosylmethionine (AdoMet) to ACC and 5'-methylthioadenosine, the committed step in ethylene biosynthesis in plants. Apple ACC synthase was overexpressed in Escherichia coli (3 mg/liter) and purified to ...

The role of bacterial 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity in the interaction between tomato (Lycopersicon esculentum=Solanum lycopersicum) and Pseudomonas brassicacearum was studied in different strains. The phytopathogenic strain 520-1 possesses ACC deaminase activity, an important trait of plant growth-promoting rhizobacteria (PGPR) that stimulates root growth. The ACC-...

Copper (Cu) is an essential element, however it’s excess into the environment causes detrimental effect on plant and risks for public health. Four Cu drought tolerant 1-aminocyclopropane-1-carboxylate (ACC) deaminase producing rhizobacteria were isolated from roots of Trifolium repens L. growing smelter contaminated soils, characterized identified based 16S rRNA gene sequencing. A consortium hi...

l h e apple ripening-related cDNA insert of clone pAP4 (C.S. ROSS, M.L. Knighton, M. Lay-Yee [1992] Plant MOI Biol 19: 231238) has previously been shown to have considerable nucleic acid and predicted amino acid sequence similarity to the insert of a tomato ripening-related cDNA clone (pTOM13) that i s known to encode the enzyme I-aminocyclopropane-I-carboxylate (ACC) oxidase (A.J. Hamilton, C....

The effect o f clopyralid pretreatment (500 g/ha) on picloram-induced ethylene, ACC (1-aminocyclopropane-l-carboxylic acid), and MACC [l-(malonylamino)-cyclopropane1-carboxylic acid] was measured in rapeseed plants that were treated with 50 or 100 g/ha o f picloram. In contrast to plants that did not receive a clopyralid pretreatment, ethylene biosyn­ thesis was significantly reduced in plants ...

In the current perusal, 12 isolates of Pseudomonas were segregated by rhizospheric soil chickpea (Cicer arietinum) Madhya Pradesh, India. Isolated test organisms characterized morphologically, biochemically and 16S rRNA gene sequencing. Out 12, one isolate designated as P4 was identified aeruginosa through sequencing, which revealed 100% homology with strains DSM 50071 NBRC 12689. The phylogene...

The root of Arabidopsis thaliana is used as a model system to unravel the molecular nature of cell elongation and its arrest. From a micro-array performed on roots that were treated with aminocyclopropane-1-carboxylic acid (ACC), the precursor of ethylene, a Small auxin-up RNA (SAUR)-like gene was found to be up regulated. As it appeared as the 76th gene in the family, it was named SAUR76. Root...

Many farmers use organic fertilizers to preserve the environment, but unfortunately, it’s not as effective chemical fertilizers. In recent years, discovery of plant growth-promoting rhizobacteria (PGPR) has gained some attraction. PGPR have abilities promote growth efficiently, including corn plants. Corn (Zea mays L.) is a staple food needed in large quantities. Our results show that bacteria ...