In many cases, secondary plant products accumulate in the large central vacuole of plant cells. However, the mechanisms involved in the transport of secondary compounds are only poorly understood. Here, we demonstrate that the transport mechanisms for the major barley (Hordeum vulgare) flavonoid saponarin (apigenin 6-C-glucosyl-7-O-glucoside) are different in various plant species: Uptake into ...

The title compound, [Mn(C(8)H(4)N(8))(2)(H(2)O)(5)]·2H(2)O, is the fourth transition metal complex containing the 1,3-di(2H-tetra-zol-5-yl)benzene ligand to be structurally characterized. The Mn^II^ cation has a distorted octahedral coordination geometry. The 1,3-di(tetra-zol-5-yl)benzene ligand is planar. All H atoms bonded to O atoms participate in hydrogen bonds, which link the mol-ecules in...

A novel series of neutral and cationic dimeric surfactants were prepared involving ketalization reaction, Williamson etherification, and regioselective oxirane ring opening with primary and tertiary alkyl amines. The critical micelle concentration (CMC), effectiveness of surface tension reduction (gamma(CMC)), surface excess concentration (Gamma), and area per molecule at the interface (A) were...

Tilia species, among which is Tilia cordata Mill. (Tiliaceae), have been used in folk medicine as anxiolytic. The hydroethanolic extract was analyzed by using liquid chromatography with mass spectrometry HPLC-DAD-ESI-MS/MS in negative ion mode, and its chemical composition was compared to flavonoids reported as anxiolytics. The major flavonoids found were: quercetin-3,7-di-O-rhamnoside, kaempfe...

An ethanolic extract and its ethyl acetate-soluble fraction from leaves of Calendula officinalis L. (Asteraceae) were found to show an inhibitory effect on amylase. From the crude extract fractions, one new phenolic acid glucoside, 6'-O-vanilloyl-β-D-glucopyranose, was isolated, together with twenty-four known compounds including five phenolic acid glucosides, five phenylpropanoids, five coumar...

3-Ketovalidoxylamine A C-N lyase and glucoside 3-dehydrogenase (G3DH), two key enzymes for valienamine synthesis, are produced by Stenotrophomonas maltrophilia. The condition of producing 3ketovalidoxylamine A C-N lyase and G3DH was optimized. Validamycin A was showed to be suitable carbon source for C-N lyase and G3DH synthesis. Maximum C-N lyase production was achieved by adding 100 ml cultur...

C. Arnaboldi,1,2 D. R. Artusa,3 F. T. Avignone III,3,* M. Balata,4 I. Bandac,3 M. Barucci,5,6 J. W. Beeman,7 F. Bellini,17,18 C. Brofferio,1,2 C. Bucci,4 S. Capelli,1,2 L. Carbone,2 S. Cebrian,8 M. Clemenza,1,2 O. Cremonesi,2 R. J. Creswick,3 A. de Waard,9 S. Di Domizio,10,11 M. J. Dolinski,14,15 H. A. Farach,3 E. Fiorini,1,2 G. Frossati,9 A. Giachero,4 A. Giuliani,2,12 P. Gorla,4 E. Guardincer...

Introduction: Epidemiological studies suggest that dietary limonoids and phytosterols offer protection from some types of cancers (1). In the present study, cancer preventive components from sour orange (Citrus aurantium L.) were isolated and identified for the first time. Isolated compounds were tested for the inhibition of human colon cancer cells (HT-29) proliferation, apoptosis. Methods: So...

Hot water extracts of Ginkgo biloba seeds were analyzed for the presence of ginkgotoxin (4'-O-methylpyridoxine) by reversed-phase liquid chromatography (LC) using methanol-0.05M KH2PO4 (1 + 9, v/v) adjusted to pH 3 as mobile phase. Detection was by fluorescence (excitation 280 nm, emission 370 nm). A straight line calibration curve was obtained for the 10-100 ng injected. After addition of beta...