purpose: the purpose of this study is to model cryptosporidiosis in laboratory animals. the parasites were inoculated into animalsand thenmultiplied. the process of proliferation was compared to controlcryptosporidiosis in humans. materials and methods: twenty-five laboratory mice (4-7 days of age) and twenty-five laboratory rats (5 days of age) were assigned to the category i while the categor...

Spleen cells from DBA/2 mice, after immunization with syngeneic P815 mastocytoma cells and Corynebacterium parvum, respond to P815 in vitro with a brisk, secondary-type generation of cytotoxic cells. This cytotoxicity is mediated by antigen-specific T-lymphocytes and correlates with resistance to in vivo challenge. This model confirms the observations of previous investigators made in semisynge...

Fifteen weaned calves (age 89-141 days) were treated with dexamethasone (0.25 mg/kg, IV) for 3 days before, the day of, and the day after inoculation with 10 colony-forming units of either Escherichia coli O157:H7 (strain 86-24, which produces Shiga toxin 2 and intimin; n = 13) or nonpathogenic E. coli (strain 123, which does not produce Shiga toxin or intimin; n = 2). All calves were necropsie...

PURPOSE To evaluate the expression of tissue inhibitor of metalloproteinases (TIMP) mRNA in corneal tissues before and at early time points (6 and 12 hours and 1, 3, and 5 days) after corneal infection with Pseudomonas aeruginosa. METHODS Ribonuclease protection assays were used to detect and quantitate TIMP mRNA expression in uninfected (wounded and unwounded) and in wounded corneas inoculat...

phytophthora root and crown rot of soybean is known as a destructive disease of soy-bean both through out the world and in iran. physiologic races of phytophthora soja were determined in this research and also a fast, accurate and simple method for inoculation of soybean to test race specific resistance. during the years　2001-4, infected soybean plants at different growth stages were collected ...

Sigmodon hispidus (cotton rat) was previously found to have antibodies against Vesicular Stomatitis Virus, New Jersey serotype (VSV-NJ) under field conditions in Costa Rica. In order to evaluate their response to the virus and potential role as reservoirs, a total of 30 adult laboratory-raised cotton rats were experimentally inoculated with 107 TCID50/ml VSV-NJ, Greentree strain, as follows: 10...

The role of rotavirus in diarrheal disease of rabbits was investigated, and a model for human rotavirus infection was established. Orogastric inoculation of 8- and 12-week-old New Zealand White rabbits with a rabbit strain of rotavirus (L:ALA:84) resulted in fecal shedding of virus for 6 to 8 days from 2 to 5 days after inoculation. Most rabbits exhibited diarrhea, coincident with the onset of ...