Cross-linked dimers of ribonuclease, added at a concentration of 0.05 mg/ml to the culture medium of hepatoma (HTC) cells, were previously shown to inhibit intracellular degradation of peroxidase taken up by endocytosis. Intracellular localization showed that endocytosed peroxidase does not reach lysosomes in dimer-treated cells. The present study shows that preloading of lysosomes with fluores...

BACKGROUND Escherichia coli is a widely studied prokaryotic system. A recent study had demonstrated that reduced growth of E. coli after extended culture in Luria-Bertani broth is a result of depletion of fermentable sugars but able to sustain extended cell culture due to the presence of amino acids, which can be utilized as a carbon source. However, this had not been demonstrated in other medi...

3-Ketovalidoxylamine A C-N lyase and glucoside 3-dehydrogenase (G3DH), two key enzymes for valienamine synthesis, are produced by Stenotrophomonas maltrophilia. The condition of producing 3ketovalidoxylamine A C-N lyase and G3DH was optimized. Validamycin A was showed to be suitable carbon source for C-N lyase and G3DH synthesis. Maximum C-N lyase production was achieved by adding 100 ml cultur...

Objective(s): Heart failure (HF) is one of the leading causes of death worldwide. Due to beneficial effects of stem cells, paracrine secretion of them has recently been used by researchers. The purpose of this study was to investigate the effects of intravenous injection (IV) of conditioned medium (CM) of human amniotic membrane-derived mesenchymal stem cell (MSC-CM) o...

Cells. HeLa cells (American Type Culture Collection, Rockville, Md.) were grown in 80 ml culture medium in Roux bottles. Cells were grown in Eagle's medium (7) which contained Hanks' balanced salt solution (1 1), 10% bovine serum, 100 @zg/mlstreptomycin sulfate, and 100 units/ml penicillin G. Bottles were incubated at 36°in an humidified atmosphere of 5% CO2 , and the cells were passed by tryp...

Because of the declining frequency of anaerobic bacteremia, routinely using half the collected blood volume for anaerobic culture has been challenged. There is no data indicating whether more clinically relevant isolates would be recovered if all or most of the given blood sample were cultured aerobically. In this two-part study, we reviewed cases of anaerobic bacteremia to determine what propo...

Strain HIL Y-9031725 was isolated from a soil sample collected in India. The strain was identified as belonging to the genus Streptomyces using the methods described by Shirling and Gottlieb.2) A loopful of mature slant culture of Streptomyces Y-9031725 was inoculated into Erlenmeyer flasks (500 ml capacity) containing 100 ml of seed medium consisting of glucose 1.5%, soyabean meal 1.5%, corn s...

CARIOGENESIS has been correlated with the presence of certain streptococci in the oral cavity and on tooth surfaces. Various studies dealing with the serological relationships of cariogenic strains resembling Streptococcus mutans (CLARKE, 1924; EDWARDSSON, 1968) have implied a certain amount of heterogeneity within this group (Tow and SHKLAIR, 1967; ZINNER and JABLON, 1968; BRATTHALL, 1970). Re...

Clostridium butyricum is a kind of anaerobic bacteria, so de-aeration is extremely important during culture. In this paper, adding sodium thiosulfate and cysteine as well as mixed culture with Saccharomyces cerevisiae were adopted in de-aeration during Clostridium butyricum culture. The experimental results showed that the optimal dosage of sodium thiosulfate was 0.004g/mL, where the biomass of...