نتایج جستجو برای: 5 وlk

تعداد نتایج: 1215766  

2014
Lijuan Fu Candace R. Guerrero Na Zhong Nicholas J. Amato Yunhua Liu Shuo Liu Qian Cai Debin Ji Seung-Gi Jin Laura J. Niedernhofer Gerd P. Pfeifer Guo-Liang Xu Yinsheng Wang

Oxidation of 5-methylcytosine in DNA by ten-eleven translocation (Tet) family of enzymes has been demonstrated to play a significant role in epigenetic regulation in mammals. We found that Tet enzymes also possess the activity of catalyzing the formation of 5-hydroxymethylcytidine (5-hmrC) in RNA in vitro. In addition, the catalytic domains of all three Tet enzymes as well as full-length Tet3 c...

Journal: :Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 2006
Kenichiro Todoroki Kousaku Ishimaru Hideyuki Yoshida Takashi Yoshitake Hitoshi Nohta Masatoshi Yamaguchi

In this paper we describe a highly sensitive and selective liquid chromatographic method for the determination of 5-methoxyindoles (5-methoxyindole-3-acetic acid, 5-methoxytryptamine, 5-methoxytryptophol, and melatonin) using a post-column technique involving electrolytic demethylation followed by fluorescence derivatization with benzylamine. We separated these compounds within 30 min by revers...

2017
Cristina Bagacean Adrian Tempescul Christelle Le Dantec Anne Bordron Audrey Mohr Hussam Saad Valerie Olivier Mihnea Zdrenghea Victor Cristea Pierre-François Cartron Nathalie Douet-Guilbert Christian Berthou Yves Renaudineau

Cytosine derivative dysregulations represent important epigenetic modifications whose impact on the clinical outcome in chronic lymphocytic leukemia (CLL) is incompletely understood. Hence, global levels of 5-methylcytosine (5-mCyt), 5-hydroxymethylcytosine (5-hmCyt), 5-carboxylcytosine (5-CaCyt) and 5-hydroxymethyluracil were tested in purified B cells from CLL patients (n = 55) and controls (...

2012
Hideharu Hashimoto Samuel Hong Ashok S. Bhagwat Xing Zhang Xiaodong Cheng

The mammalian thymine DNA glycosylase (TDG) is implicated in active DNA demethylation via the base excision repair pathway. TDG excises the mismatched base from G:X mismatches, where X is uracil, thymine or 5-hydroxymethyluracil (5hmU). These are, respectively, the deamination products of cytosine, 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC). In addition, TDG excises the Tet prote...

2014
Debin Ji Changjun You Pengcheng Wang Yinsheng Wang

Recently 5-hydroxymethyl-2'-deoxycytidine (5hmdC), 5-formyl-2'-deoxycytidine (5fdC), and 5-carboxyl-2'-deoxycytidine (5cadC) were discovered in mammalian DNA as oxidation products of 5-methyl-2'-deoxycytidine (5mdC) induced by the ten-eleven translocation family of enzymes. These oxidized derivatives of 5mdC may not only act as intermediates of active cytosine demethylation in mammals but also ...

2014
Maria Chudnovsky Louis Esperet Laetitia Lemoine Peter Maceli Frédéric Maffray Irena Penev

We prove that a graph G contains no induced 5-vertex path and no induced complement of a 5-vertex path if and only if G is obtained from 5-cycles and split graphs by repeatedly applying the following operations: substitution, split unification, and split unification in the complement (where split unification is a new class-preserving operation that is introduced in this paper).

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